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目的 :可为HPV16L1重组蛋白疫苗及诊断试剂盒的研制打下基础。方法 :含有HPV16L1pPIC3.5重组质粒的GS115阳性菌株 ,经甲醇诱导在甲醇营养型酵母菌中表达HPV16L1蛋白 ,经SDS PAGE电泳 ,对所表达蛋白进行分析、鉴定。结果 :HPV16L1 pPIC3.5重组质粒经甲醇诱导产生 5 5KD大小的L1靶蛋白 ,经SDS PAGE电泳表明 ,诱导第四天L1蛋白表达量最大。结论 :甲醇可诱导HPV16L1靶蛋白的表达 ,并且在第四天表达量最大。
Objective: To lay the foundation for the development of HPV16 L1 recombinant protein vaccine and diagnostic kit. Methods: The positive strain of GS115 containing HPV16L1pPIC3.5 recombinant plasmid was induced by methanol to express HPV16L1 protein in methanolic yeast. The protein was analyzed by SDS PAGE and identified. Results: HPV16L1 pPIC3.5 recombinant plasmid was induced by methanol to produce L1 target protein of 5 5KD size. SDS PAGE electrophoresis showed that the expression of L1 protein reached the maximum on the fourth day. Conclusion: Methanol can induce the expression of HPV16 L1 target protein and express the highest level on the fourth day.