论文部分内容阅读
以20份羽衣甘蓝材料为试材,采用游离小孢子培养的方法,研究了胚胎发生、胚状体成苗和丛生芽继代培养的影响因素。结果表明:在诱导胚胎发生过程中,基因型是关键因素,供试材料中有15份诱导出胚,诱导率为75%;最适蔗糖浓度也与基因型有关;在不同基因型中,6-BA与NAA最佳浓度配比为1∶1或2∶1。与B5相比,MS培养基更有利于胚状体成苗。丛生芽转接周期为15d时增殖效果最好,平均增殖系数为10.3。
Twenty parts of kale were used as materials to study the factors affecting embryogenesis, embryogenesis and subculture of cluster buds by using the method of free microspore culture. The results showed that genotype was the key factor in inducing embryogenesis. 15 of the tested materials were inducible, with the induction rate of 75%. The optimum sucrose concentration was also related to genotype. Among the genotypes, 6 -BA and NAA optimal concentration ratio of 1: 1 or 2: 1. Compared with B5, MS medium is more conducive to embryoid body seedling. The best proliferative effect was achieved when the cluster shoots transferred 15 days and the average multiplication coefficient was 10.3.