目的:建立宫血宁胶囊中总皂苷的含量测定方法,方法:采用可见分光光度法.以薯蓣皂苷元为对照,采用高氯酸反应显色,测定波长408nm。结果:薯蓣皂苷元在0.002816～0.01971mg/mL浓度范围内与吸光度呈良好的线性关系,r=0.9992。回收率为101.6%,RSD为2.9%(n=5)。结论:该方法简便快速,灵敏度高,重复性好,适用于该制剂的质量控制。 Objective: To establish a method for determination of total saponins in Gongxuening Capsules. Methods: Spectrophotometry was used. Diosgenin was used as control, and the color was determined by perchloric acid reaction. The wavelength was measured at 408 nm. Results: There was a good linear relationship between the absorbance and the absorbance of diosgenin in the concentration range of 0.002816-0.01971mg/mL, r=0.9992. The recovery was 101.6% and the RSD was 2.9% (n=5). Conclusion: The method is simple, rapid, sensitive, and reproducible. It is suitable for the quality control of the preparation.