高氧预适应后大鼠抗氧化酶活性及肺组织的变化(英文)

来源 :中国临床康复 | 被引量 : 0次 | 上传用户:IDYLL123
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
背景:连续高浓度吸氧可以提高大鼠抗氧化酶,同时对肺产生损伤作用。目的:观察间断、高浓度吸氧对大鼠抗氧化酶活性及肺组织的影响。设计:完全随机设计,对照实验。单位:解放军总医院老年心内科和生化科。材料:实验于2002-01/2002-05在海军总医院高压氧科和解放军总医院生化科内完成。选用健康Wister大鼠10只,体质量200~250g,雌雄不拘。随机将大鼠分为2组,每组5只。方法:对照组大鼠在室内环境喂养7d。高氧预适应组动物放入高压氧仓内,每日吸80%~85%的氧气(101.325kPa)6h,连续7d。实验结束后取大鼠肺称肺湿重。并取肺组织0.4g,在冰浴上制成匀浆,取上清液采用化学发光法分别测定超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性。将肺组织采用30g/L戊二醛固定,制成切片后电镜下观察肺脏超微结构。主要观察指标:①两组大鼠肺湿重及肺组织超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性比较。②肺组织超微结构改变。结果:大鼠10只均进入结果分析。①肺组织超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶活性:高氧预适应组均明显高于对照组[(185.10±1.92)%/g,(90.50±4.17)nkat/g,(4.38±0.18K/g;(95.15±1.43)%/g,(20.17±2.17)nkat/g,(1.10±0.19)K/g,t=-84.016,-33.488,-28.023,P<0.01]。②大鼠肺湿重:高氧预适应组与对照组相近[(1.32±0.07),(1.30±0.03)g,P>0.05],说明高氧预适应组肺内液体没有增加。③肺组织超微结构:高氧预适应组肺组织基膜完整、厚度均匀一致,血管内皮细胞完整,Ι型上皮细胞无破坏。结论:间断、高浓度吸氧能提高大鼠肺内抗氧化酶活性,对大鼠肺无明显的损伤作用。 Background: Continuous high concentration of oxygen can increase rat anti-oxidant enzymes, while damaging the lungs. Objective: To observe the effects of intermittent and high concentration oxygen on antioxidant enzyme activity and lung tissue in rats. Design: completely random design, control experiment. Unit: Department of Geriatrics, General Hospital of People’s Liberation Army and Department of Biochemistry. MATERIALS: The experiment was performed in the Department of Biochemistry of the Navy General Hospital and PLA General Hospital from January 2002 to May 2002. Selection of healthy Wister rats 10, weight 200 ~ 250g, male or female. Rats were randomly divided into two groups of five. Methods: The control group rats were fed in the indoor environment for 7 days. Hypoxic preconditioning group of animals into the hyperbaric oxygen chamber, inhaling 80% to 85% oxygen (101.325kPa) 6h, 7d consecutive days. After the experiment, the lungs of rats were weighed and weighed. 0.4g of lung tissue was taken and homogenized in an ice bath. The supernatants were assayed for superoxide dismutase, glutathione peroxidase and catalase activity by chemiluminescence. The lung tissue was fixed with 30g / L glutaraldehyde, and the lung ultrastructure was observed under electron microscope. MAIN OUTCOME MEASURES: ① lung wet weight and lung tissue superoxide dismutase, glutathione peroxidase and catalase activity in two groups were compared. ② lung ultrastructure changes. Results: Ten rats were involved in the result analysis. (1) The activities of superoxide dismutase, glutathione peroxidase and catalase in lung tissue were significantly higher in hyperoxia preconditioning group than those in control group [(185.10 ± 1.92)% / g, (90.50 ± 4.17) nkat / g, (4.38 ± 0.18 K / g; 95.15 ± 1.43% / g, 20.17 ± 2.17 nkat / g, 1.10 ± 0.19 K / g, t = -84.016, -33.488, -28.023, P <0.01] .②The lung wet weight of rats: The Hypoxic preconditioning group was similar to the control group [(1.32 ± 0.07), (1.30 ± 0.03) g, P> 0.05] Increased.③The ultrastructure of lung tissue: The preconditioning group had a complete basement membrane and uniform thickness, the vascular endothelial cells were intact, and the type I epithelial cells were not damaged.Conclusion: Intermittent and high concentration of oxygen can improve the intrapulmonary Antioxidant enzyme activity, no significant damage to rat lung.
其他文献
世人谁能相信?1980年一支只有40余人的乡镇施工队,如今已发展成为拥有6000余名职工,多业并举、能打硬仗的综合性建筑施工企业。这就是江苏省如皋市第二建筑安装工程公司。该公司
金钱草[1]为报春花科珍珠菜属植物过路黄的干燥全草,目前广泛用于湿热黄疸、痈肿疮疡、毒蛇咬伤、毒蕈及药物中毒和肝胆结石、尿路结石等疾病的治疗。金钱草品种众多,同名异
北京市地方税务综合服务管理系境自今年4月1日运行以来,给纳税人带来了许多便利,其中,税银联网的缴税方式更是一经推出就在北京的高新技术核心地带——海淀区,受到了广大纳
钢产品的生产工艺从节能的角度看已经发生了很大变化,最近又采用了热装轧制工艺(HCR)HCR工艺是一个将轧制工艺和连铸工艺联系到一起的新轧制工艺。在此工艺中,连铸板坯不用
2003年,青岛各级财税部门面对“非典”疫情和各种减收增支因素带来的不利影响,知难而进,依法组织收入,大力增收节支,有力地支持了全市改革、经济建设和各项事业发展。全市地
人不是斯氏狸殖吸虫的适宜宿主,幼虫寄生后可引起多种肺外型感染,病原学检查困难[1],临床诊断主要依靠免疫学检查,除检测方法外,诊断用抗原的特异性是影响检测效果的关键因素
最近,我们发现一些外地企业以上门送货、上门送票、现金结算的方式与本辖区纳税人洽谈生意,经发函协查,取得的这些增值税专用发票近一半为虚开发票,致使本辖区19户企业取得
2011年第12期《集邮博览》刊登的“漏印英文加注的《迎客松》普资片”一文,展示了一枚漏印英文“CHINA”的2001年6月28日发行的《迎客松》普资片加印片。先看《迎客松》普资
请下载后查看,本文暂不支持在线获取查看简介。 Please download to view, this article does not support online access to view profile.
期刊
近日,美术基金(Fine Art Fund Group)首席执行官菲利普·霍夫曼(Philip Hoffman)做出预测:在未来的10年中,富有人群中拥有艺术品人群比例将由目前的5%上升至40%如果以凯捷美