论文部分内容阅读
目的观察血浆中sLOX-1浓度是否和LOX-1基因多态性相关。方法采用酶联免疫吸附法测定354例接受冠状动脉造影的浙东地区汉族人群住院患者血清中sLOX-1的浓度,并采用Tm-shift基因分型方法对G501C和3’UTR-C/T等位基因型进行检测,其中包括43例急性心肌梗死、86例不稳定型心绞痛、89例稳定型心绞痛和136例经冠状动脉造影排除冠心病的患者。结果 3’UTR-C/T位点TT,TC,CC基因型分别为5.4%,37.3%,57.3%,501G/C位点CC,GC,GG基因型分别为5.4%,31.9%,62.7%,3’UTR-C/T TT,TC,CC 3组sLOX-1浓度分别为160.0±56.1,132.7±52.0,128.9±58.0pg/ml,501G/C CC,GC,GG 3组sLOX-1浓度分别为134.0±55.2,127.2±50.8,134.2±58.7pg/ml。不同基因型患者sLOX-1浓度各组间没有显著性差异,在各亚组间也没有差异。结论浙东地区汉族人群sLOX-1浓度和LOX-1基因多态性无相关性。
Objective To observe whether the concentration of sLOX-1 in plasma is related to the polymorphism of LOX-1 gene. Methods Serum concentration of sLOX-1 was measured in 354 hospitalized patients with coronary angiography in eastern Zhejiang province by enzyme-linked immunosorbent assay. The levels of sLOX-1 in G501C and 3’UTR-C / T were determined by Tm-shift genotyping Genotypes were detected, including 43 cases of acute myocardial infarction, 86 cases of unstable angina, 89 cases of stable angina pectoris and 136 cases of patients undergoing coronary angiography to exclude coronary heart disease. Results The genotypes of TT, TC and CC in 3’UTR-C / T loci were 5.4%, 37.3% and 57.3%, respectively. The CC, GC and GG genotypes at the site of 501G / C were 5.4%, 31.9% and 62.7% , And the concentrations of sLOX-1 in 3’UTR-C / T TT, TC and CC 3 groups were 160.0 ± 56.1,132.7 ± 52.0,128.9 ± 58.0pg / ml and 501G / C CC, 134.0 ± 55.2, 127.2 ± 50.8, 134.2 ± 58.7 pg / ml, respectively. There was no significant difference between groups for sLOX-1 concentrations in different genotypes, nor was there any difference between subgroups. Conclusion There is no correlation between the concentration of sLOX-1 and the polymorphism of LOX-1 gene in eastern Han population in Zhejiang Province.