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应用RAPD与dpRAPD方法鉴定萝卜-甘蓝型油菜中萝卜基因组,筛选了140条随机引物。结果表明,平均每条引物(组合)能产生的萝卜基因组特异标记数dpRAPD高于RAPD,分别为1.69和1.33;在dpRAPD扩增产物中有77.6％谱带清晰易辨,略高于RAPD(75.4％)。两者所检测到的萝卜基因组标记大部分为各自特异的扩增产物。由于结合了荧光标记引物,dpRAPD反应产物可在Genetic Analyzer上分离检测,因此能检测到100 bp以下的小片段DNA。
RAPD and dpRAPD were used to identify radish genomes in radish - Brassica napus and 140 random primers were screened. The results showed that the average number of genomic tagged dpRAPDs per radicle per primer was higher than that of RAPD (1.69 and 1.33, respectively), 77.6% of the amplified bands were clearly identified, which was slightly higher than that of RAPD (75.4 %). Most of the radish genomic markers detected by the two are their own specific amplification products. Due to the incorporation of fluorescently labeled primers, the dpRAPD reaction product can be separated and detected on a Genetic Analyzer and therefore can detect small fragments of DNA up to 100 bp.