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目的 :观察诱导分化剂二甲基甲酰胺 (DMF)对胶质瘤细胞C6的增殖抑制作用 ,探讨作用机理中DMF对c myc、p16基因蛋白水平表达的影响。方法 :[3 H] TdR掺入法测增殖抑制率 ;免疫组化SP法测C myc、p16基因蛋白水平的表达。结果 :应用二甲基甲酰胺后 ,胶质瘤细胞CPM值降低 ,DNA合成总量减少。C myc基因胞浆阳性表达下降 ,p16基因胞浆阳性表达升高。结论 :二甲基酰胺对胶质瘤细胞增殖存在剂量依赖性抑制。作用机理与降低了癌基因C myc对C6细胞恶性增殖的转录调节作用、增强了抑制基因p16活性有关
OBJECTIVE: To observe the inhibitory effect of dimethyl formamide (DMF) on the proliferation of C6 glioma cells and to explore the effect of DMF on the expression of c-myc and p16 genes in the mechanism of action. Methods: [3H] TdR incorporation method was used to measure the inhibition rate of proliferation. The expression of C-myc and p16 protein was detected by immunohistochemical SP method. Results: After using dimethylformamide, the CPM value of glioma cells decreased and the total amount of DNA synthesis decreased. Cytoplasm positive expression of C myc gene decreased, p16 gene cytoplasmic positive expression increased. Conclusion: Dimethylamide inhibits the proliferation of glioma cells in a dose-dependent manner. Mechanism and reduce the oncogene C myc C6 malignant proliferation of transcriptional regulation, increased inhibition of gene p16 activity