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本研究使用两种大型真菌茶树菇新型凝集素AAL(Agrocybe aegerita lectin)和AAL2(Agrocybe aegerita lectin 2),以检测小鼠CD8~+T淋巴细胞表面糖基化。用生物素标记的AAL和AAL2结合流式细胞仪,分别分析从C57BL/6小鼠脾脏和腹股沟淋巴结分离出来的CD8~+T淋巴细胞表面的糖基化水平,及其细胞因子表达水平。结果发现脾脏中AAL~+CD8~+,AAL2~+CD8~+T淋巴细胞所占的比例(%)分别为6.72±3.00和12.18±5.28,而腹股沟淋巴结的AAL~+CD8~+,AAL2~+CD8~+T淋巴细胞所占的比例(%)分别为12.18±5.28和10.15±3.46。细胞因子表达水平结果发现小鼠脾脏AAL2~+CD8~+T细胞表达perforin的细胞比例低于AAL2-CD8~+T细胞(p<0.05,有统计学意义),腹股沟淋巴结中AAL~+CD8~+T细胞表达perforin的细胞比例则高于AAL-CD8~+T细胞(p<0.05,有统计学意义)。该数据为研究小鼠脾脏和腹股沟淋巴结CD8~+T细胞表面糖基化提供了新的数据,也为两种新型凝集素AAL和AAL2的应用提供理论依据。
In this study, two large fungal Agrocybe aegerita lectin (AAL) and AAL2 (Agrocybe aegerita lectin 2) were used to detect the surface glycosylation of mouse CD8 + T lymphocytes. Biotinylated AAL and AAL2 were combined with flow cytometry to analyze the glycosylation level of CD8 + T lymphocytes isolated from the spleen and inguinal lymph nodes of C57BL / 6 mice and the expression of cytokines. The results showed that the percentage (%) of AAL ~ + CD8 ~ + and AAL2 ~ + CD8 ~ + T lymphocytes in the spleen were 6.72 ± 3.00 and 12.18 ± 5.28 respectively, while the AAL ~ + CD8 ~ + CD8 ~ + T lymphocytes (%) were 12.18 ± 5.28 and 10.15 ± 3.46, respectively. The results of cytokine expression showed that the percentage of cells expressing perforin in AAL2 ~ + CD8 ~ + T cells in spleen was lower than that in AAL2-CD8 ~ + T cells (p <0.05, statistically significant) + T cells perforin cells than the proportion of AAL-CD8 ~ + T cells (p <0.05, statistically significant). The data provide new data for studying the surface glycosylation of CD8 ~ + T cells in spleen and inguinal lymph nodes in mice and provide theoretical basis for the application of two new lectins AAL and AAL2.