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利用来自抗旱性较好的供体亲本(BG300和BG304)、具有两种遗传背景(IR64和特青)的水稻高代回交(BC2)抗旱选择导入系,通过人工接种的方法鉴定纹枯病抗性,考察纹枯病抗性与抗旱性之间可能存在的遗传重叠。通过与受体亲本的纹枯病抗性表现比较发现,具有特青背景的抗旱选择导入系倾向于纹枯病抗性的降低,而IR64背景的抗旱选择导入系则倾向于纹枯病抗性的增强。基于基因型与表型的方差分析共鉴测到6个与纹枯病抗性相关的位点,其中QSbr6在不同供体和背景的两个群体中分别检测到,而QSbr10则在同一供体的两个遗传背景下均检测到;有3个位点(QSbr6、QSbr8和QSbr10)与同一群体中检测到的抗旱性位点位置相近,很可能是两种抗性重叠的遗传基础。尽管方差分析的方法在选择导入系的非选择目标性状相关位点的鉴定中存在相当程度的偏低估计,本研究所检测到的纹枯病抗性位点,特别是那些与抗旱性重叠位点的分子标记以及相关的抗性株系仍将为进一步的水稻纹枯病抗性和抗旱性的多抗性育种和深入的遗传重叠研究提供有用的信息和材料。
The resistance to drought of rice (BC2) with two genetic backgrounds (IR64 and Teqing) was selected from the donor parents (BG300 and BG304) with better drought resistance, and the sheath blight was identified by artificial inoculation Resistance, to examine the possible overlap of resistance between sheath blight resistance and drought resistance. Compared with the sheath blight resistance of the recipient parent, drought-resistant selection lines with Teqing background tended to reduce the resistance to sheath blight, whereas the drought-resistant selection lines with IR64 background tended to have sheath blight resistance Enhanced. A total of 6 spots related to resistance to sheath blight were detected using ANOVA analysis of genotypes and phenotypes. QSbr6 was detected in two populations from different donors and backgrounds, while QSbr10 was found in the same donor (QSbr6, QSbr8 and QSbr10) were similar to those detected in the same population, which is likely to be the genetic basis of the two resistance overlaps. Although the method of ANOVA showed that there was a considerable degree of low estimation in the identification of the loci related to the non-selected traits of the selected inbred lines, the resistances detected in this study were especially susceptible to drought resistance Dot molecular markers and related resistant lines will provide useful information and materials for further multiresistant breeding and further genetic overlap studies on sheath blight resistance and drought resistance in rice.