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采用标记链霉菌素-生物素免疫酶技术结合微波炉处理方法,对15例成人型喉乳头状瘤,10例喉部不典型增生,2例喉原位癌,10例声带息肉以及6例正常喉的组织标本进行了检测,结果发现:在73.3%(11/15)喉乳头状瘤、40%(4/1f)喉不典型增生和50%(1/2)喉原位癌中检测到p53蛋白过度表达,而10例声带息肉和6例喉部正常组织无一例出现p53的过度表达。提示:p53过度表达是喉癌发生中的早期事件,而p53的过度表达可能为具有高度癌变危险的癌前病提供早期诊断的辅助指标。
Fifteen cases of adult laryngeal papilloma, 10 cases of atypical hyperplasia of the larynx, 2 cases of laryngeal carcinoma of the larynx, 10 cases of vocal cord polyps and 6 cases of normal laryngeal polyps were treated with the labeled streptomycin-biotin-immunoenzyme technique combined with microwave oven treatment. Of the tissue specimens were detected and found that p53 was detected in 73.3% (11/15) of the laryngeal papilloma, 40% (4 / 1f) of the atypical hyperplasia of the larynx and 50% of the laryngeal carcinoma in situ Overexpression of p53, while 10 cases of vocal cord polyps and 6 cases of laryngeal normal tissue without p53 overexpression. It is suggested that overexpression of p53 is an early event in the development of laryngeal carcinoma, while overexpression of p53 may be an auxiliary indicator of early diagnosis of precancerous lesions with a high risk of canceration.