patch-clamp相关论文
Substance P (SP),the first identified neuropeptide,has long been indicated to be involved in the vagal control of heart ......
Activation of medullary α2-adrenoceptors in vitro does not disinhibit laryngeal motoneurons: failure
The preganglionic recurrent laryngeal motoneurons (PRLMs) dominate the neural control of almost all the intrinsic laryng......
Somatostatin receptor-mediated suppression of GABAergic synaptic transmission in cultured rat retina
Somatostatin(SRIF)is widely distributed in the central nervous system,which may modulate neurotransmitter release via ac......
Firing properties of identified airway-related preganglionic parasympathetic neurons in the ventrola
The airway function is dominated by the parasympathetic nervous system.A subpopulation of the airway-related preganglion......
Activation of medullary α1A-adrenoceptors increases airway vagal tone via enhanced excitatory inputs
In mammals including the human beings, the neural control of airway smooth muscles is dominated by medullary airway vaga......
Activation of medullary α2-adrenoceptors in vitro does not disinhibit laryngeal motoneurons: failure
The preganglionic recurrent laryngeal motoneurons (PRLMs) dominate the neural control of almost allthe intrinsic larynge......
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Electrical remodeling of membrane ionic channels of hypertrophied ventricular myocytes from spontane
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Miniature endplate potentials (MEPPS) caused by the spontaneous release of ACh from the growth cone of cholinergic neuro......
Using the whole cell patch-clamp technique,we studied the effects of La3+ on calcium-activated K+ currents and its kinet......
In recent years the evolution of olfactory bulb periglomerular cells,as well as the function of periglomerular cells in ......
目的:研究硫氧还蛋白(Trx)系统对糖尿病(DM)大鼠左室心肌细胞瞬时外向钾电流(Ito)的影响。方法:以链脲菌素(STZ)诱导的DM大鼠模型......
目的 研究不同浓度胰岛素对家兔右室心肌细胞L型钙电流(ICa-L)的影响.方法 采用膜片钳技术记录家兔右室心肌的ICa-L,予150、400和1......
,Effects of Simvastatin on Ion Channel Currents in Ventricular Myocytes from Acute Infarcted Heart o
Objectives To investigate the effects of simvastatin on membrane ionic currents in left ventricular myocytes of rab-bit ......
,Na+ Channels Activation Recovery from Use-dependent Block in Human Embryonic Kidney Cells and Effec
Objectives This experiment used whole-cell patch-clamp technique to investigate the course of recovery from use-dependen......
Aim:To examine the effects of ginseng saponins on the heterologously expressedhuman ether-a-go-go related gene(HERG)tha......
Aim: To investigate the effect of aluminum (Al) on high voltage-dependent calcium current (IHVA) and its modulation by g......
,Pharmacological and functional comparisons of α6/α3β2β3-nAChRs and α4β2-nAChRs heterologously expre
Neuronal nicotinic acetylcholine receptors containing α6 subunits (α6*-nAChRs) show highly restricted distribution in ......
Cocaine is one of the most abused illicit drugs worldwide.It is well known that the dopamine (DA) transporter is its maj......
目的 构建钙调蛋白(CaM)的N末端片段(N-lobe)、C末端片段(C-lobe)及其钙离子(Ca2+)结合位点突变体(N-lobe12,C-lobe34)原核表达载......
Ettects of simvastatin on ion channel currents in ventricular myocytes from rabbit with acute myocar
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Effects of BaCl2 on slow vacuolar ion channels on radish by patch-clamp YANG Pin (杨 频) & ZHANG Liping (张丽平) Insti......
目的 研究米诺环素对大鼠心室肌细胞L型钙电流(Ica-L)动力学特性的影响.方法 以酶解法分离获得单个心室肌细胞,采用全细胞膜片钳技......
Objective To investigate the effects of BmkTXKβ, a newly purified ‘long chain peptide inhibitor of K+ channels from th......
The effects of a new O-superfamily conotoxin, SO3, on sodium current (/Na), transient A-type potassium currents (/A), an......
The properties of the inward current of medulla terminalis-X-organ (MTXO) cells isolated from the Penaeusjaponicus eyest......
本文提出了一种基于ARM9处理器和Linux操作系统的嵌入式膜片钳解决方案。文章首先介绍了系统的整体结构．接着介绍了嵌入式Linux系统......
目的建立稳定的成年犬心房肌细胞的分离方法,进行膜片钳实验的研究。方法采用Langendorff灌流,台氏液灌流10s后无钙台氏液灌流5min......
应用机械分离技术获得豚鼠单脑初级精母细胞后，进行全细胞式膜片钳记录。结果表明；在标准盐溶液中，当钳制电位－９０ｍＶ，指令电压（－６０￣＋１４０）ｍＶ，步阶电压２０ｍＶ时，可记......
Roles of the ocular pressure, pressure-sensitive ion channel, and elasticity in pressure-induced ret
The intraocular pressure inside the human eye maintains 10–21 mmHg above the atmospheric pressure.Elevation of intraocu......
目的观察ghrelin对GH3细胞膜电位的作用.方法用Nystatin打孔全细胞记录跨膜电流,观察ghrelin组和对照组GH3细胞膜电位.结果对照组......
从剖析膜片钳实验的技术要求出发，介绍了国产第二代膜片钳放大器中的关键电路，即电容补偿电路、串联电阻预测与补偿电路和探头高阻切......
目的：探讨心肌梗死后大鼠左心室肌细胞钾通道的重构及增加葡萄糖代谢对钾流的作用。方法：取体重200—250g的雄性Sprague—Dawley（SD）大......
目的研究肾脏髓袢升支粗段管周膜钾通道的类型及其电流特征。方法应用单通道膜片钳技术记录大鼠肾脏髓袢升支粗段管周膜钾通道电流......
目的:本文研究非选择性M受体激动剂氨甲酰胆碱(Cch)对心肌细胞收缩的作用,并同时观察L-型钙流,探讨其机制.方法:以分离大鼠的单个......
目的：建立一种适合膜片钳单通道记录的脊髓背根神经节神经元急性分离方法。方法：用酶消化和机械分离相结合的方法急性分离大鼠DRG神......
目的观察葛根素提取液对豚鼠乳头肌动作电位及延迟整流钾电流的影响．方法用常规微电极方法记录豚鼠乳头肌动作电位，用全细胞膜片钳技......
目的 通过研究辛伐他汀预处理对免心肌缺血再灌注后L型钙离子通道电流（ⅠCa-L）的影响，探讨他汀类药物抗心律失常的细胞学离子机制。方......
目的：研究乙醇对低分化鼻咽癌CNE-2Z细胞氯通道的影响并探讨其分子机制。方法：MTT检测乙醇对细胞生长的影响；全细胞膜片钳技术记录乙......
目的：探讨催产素在脊髓后角胶状质细胞层的镇痛机制。方法：本研究使用成年雄性SD大鼠,运用椎板切除术取出脊髓腰骶膨大节段（L1~S3）,并......
目的记录幼年非洲爪蟾视顶盖区第六层神经元的自发性微突触后电流(mPSCs).方法应用盲法电压膜片钳全细胞记录技术.结果观察到用谷......
运用电生理膜片钳技术，对麻黄碱作用于HCN通道的药理学机制进行了研究，发现了麻黄碱对HCN通道电流有一定的抑制作用．......
目的:建立果蝇幼虫中枢神经元细胞培养方法,为研究吸入麻醉药相关的离子通道建立实验模型.方法:取晚期三龄幼虫的脑腹神经节经解剖......
目的已知特发性室速主要起源于右室流出道（RVOT）,由于技术上的困难,目前对特发性右室流出道室速（RVOT-VT）的离子通道机制研究很少,本实......
电生理研究细胞分泌有两种实时记录技术.膜电容法通过记录细胞膜表面积来估算刺激诱导的细胞分泌和胞吞导致的膜表面积总和变化.微......
In this study, whole cell patch clamp recording technique was employed to investigate the effect of Shenmai Injection (S......
针对膜片钳实验系统自动化发展的需要§线，研究并实现了一种新的数据采集系统——UDA-I采用基于CPLD芯片的数字逻辑控制单元和U......
以拟南芥 ArabidopsisthalianaColumbia 根为材料,利用膜片钳技术测定其根细胞原生质体质膜内向K+电流,并对Na+对其K+电流的影响进......
为了解细胞膜氢离子泵的电生理特性,本实验用膜片箝法研究了大麦根木质部薄壁细胞质膜上的生电泵在无一价阳离子条件下的生电活性......
