retrovirus相关论文
In vertebrates,perception of sound,motion,and balance is mediated through sensorineural hair cells and spiral ganglion n......
Differentiation of NIH3T3 fibroblasts into adipocytes induced by peroxisome proliferator activated r
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Expression and biological activity of double replica retrovirus carrier-mediated neurotrophin-3 in o
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To date, it remains poorly understood whether astrocytes can be easily reprogrammed into neurons. Mash1 and 2 have been ......
目的 构建表达重组人骨形成蛋白2(BMP2)基因的重组逆转录病毒,对其在成骨细胞中的生物学作用进行探讨.方法 克隆BMP2基因,与pDNR-C......
目的探讨逆转录病毒介导人血管内皮抑制素基因(endostatin)对人肝癌细胞的生长抑制作用.方法利用逆转录病毒载体pLncx构建含有人en......
目的:观察重组逆转录病毒人血管内皮生长因子165(retrovirus pLXSN/hVEGF165)基因转染对人骨髓基质细胞(hM-SCs)成骨能力的影响。......
目的应用小鼠L6565白血病病毒建立小鼠艾滋病模型,并在此模型上观察抗艾滋病药物双汰芝的治疗作用.方法用小鼠L6565白血病病毒感染......
,Transfection of promyelocytic leukemia in retrovirus vector inhibits growth of human bladder cancer
Aim: To construct a recombinant retrovirus vector carrying human promyelocytic leukemia (PML) cDNA and identify its expr......
本文通过对荣华二采区10...
Objective: To establish a retroviral-mediated suicide gene therapy system for experimental glioma and test its efficacy.......
Objective: To establish an efficient and safe gene transfer system mediated by retrovirus for gene marking and gene ther......
RETROVIRAL MEDIATED EFFICIENT TRANSFER ANDEXPRESSION OF MULTIPLE DRUG RESISTANCE GENE TO HUMAN LEUKE
Objective: To investigate retroviral-mediated transfer and expression of human multidrug resistance (MDR) gene MDR1 in l......
Effect of local viral transfer of interleukin 10 gene on a rabbit arthritis model induced by interle
Backgroud Interleukin 1β(IL-1 β)is the principal mediator in the pathogenesis of rheumatoid arthdtis.Continuous inject......
Osteogenic potential of human calcitonin gene-related peptide alpha gene-modified bone marrow mesenc
Background Most of the basic and clinical studies of osteonecrosis of the femoral head (ONFH) are restricted to bone tis......
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Construction and expression of retroviruses encoding dual drug resistance genes in human umbilical c
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Effect of the control proliferation of astrocyte on the formation of glial scars by antisense GFAP r
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Specific targeted killing of ErbB2 positive breast cancer by retrovirus-mediated Immunocaspase-3 sec
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Expression and biological activity of double replica retrovirus carrier-mediated neurotrophin-3 in o
BACKGROUND: Previous studies have demonstrated that the combination of olfactory ensheathing cells (OECs) and neurotroph......
病毒感染与癌症的关系是生物医学领域中非常重要的研究方向,估计当前全世界至少20%人类肿瘤的发生与病毒感染有密切联系.本文对反......
RETROVIRAL MEDIATED EFFICIENT TRANSFER ANDEXPRESSION OF MULTIPLE DRUG RESISTANCE GENE TO HUMAN LEUKE
Objective: To investigate retroviral-mediated transfer and expression of human multidrug resistance (MDR) gene MDR1 in l......
目的 通过逆转录病毒介导两种类型人干细胞因子在NIH3T3细胞中稳定表达,并研究它们对白血病细胞的作用.方法 用DNA重组技术构建并......
Specific targeted killing of ErbB2 positive breast cancer by retrovirus-mediated Immunocaspase-3 sec
In this study, Immunocaspase-3 gene was transfected into Jurkat T lymphocytes and the targeted proapoptotic protein Immu......
天然免疫分子APOBEC3G蛋白酶是一种胞苷脱氨酶,是机体固有免疫反应的新成员,参与宿主抵抗病毒入侵的天然免疫防御机制,具有较强的......
目的 为了获得通用型逆转录病毒载体.方法 本实验在成功构建复制缺陷型逆转录病毒载体的基础上,对复制缺陷型逆转录病毒载体的包装......
Objective:The transcriptional repressor Pokemon(encoded by the Zbtb7 gene)is a critical factor in oncogenesis.Pokemon ov......
Effect of local viral transfer of interleukin 10 gene on a rabbit arthritis model induced by interle
Backgroud Interleukin 1β(IL-1 β)is the principal mediator in the pathogenesis of rheumatoid arthdtis.Continuous inject......
Osteogenic potential of human calcitonin gene-related peptide alpha gene-modified bone marrow mesenc
Background Most of the basic and clinical studies of osteonecrosis of the femoral head (ONFH) are restricted to bone tis......
Construction and expression of retroviruses encoding dual drug resistance genes in human umbilical c
A series of retroviral vectors encoding human mdr1 gene alone as well as in combination with either human mgmt gene or h......
ｃ－ｍｙｃ第一外显子在ｃ－ｍｙｃ转录调节中重要作用。为了解ｃ－ｍｙｃ第一外显子的生物学效应，探讨ｃ－ｍｙｃ的转录调控机制，为ｃ－ｍｙｃ表达增高性疾病的基因治疗提供依据。我们采......
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目的：构建针对趋化因子受体CXCR4的RNA干扰逆转录病毒载体，检测由逆转录病毒介导的RNA干扰对人宫颈癌Caski细胞CXCR4基因表达的抑制......
ＥＸＰＥＲＩＭＥＮＴＡＬＳＴＵＤＩＥＳＯＮＲＡＤＩＡＴＩＯＮ－ＩＮＤＵＣＩＢＬＥＨＵＭＡＮＴＮＦＧＥＮＥＴＨＥＲＡＰＹＦＯＲＣＡＮＣＥＲＣａｏＸｕｅｔａｏ曹雪涛ＺｈａｎｇＷｅｉｐｉｎｇ章卫平ＷａｎｇＪｉａｎｌｉ王建莉ＨｕａｎｇＸｉｎ黄欣ＱｉｎＺｈｉｈ.........
目的:观察人类野生型p53(wt p53)基因对人食管癌细胞系的抑制作用. 方法:用逆转录病毒为载体,将外源性wt p53基因导入人食管癌细胞......
目的:探讨凋亡相关基因Bcl-2过表达对抗过氧化氢诱导的雪旺细胞凋亡的影响.方法:构建含有人Bcl-2cDNA的逆转录病毒真核表达载体PLX......
①目的比较逆转录病毒载体4种转染方法对小鼠脾脏T淋巴细胞的转染率.②方法应用衣霉素预处理包装细胞提高逆转录病毒滴度,运用标准......
目的建立可表达随机12肽库的逆转录病毒初级文库的构建方法并优化其实验参数。方法体外合成可编码随机12肽的DNA片段。将该DNA片段......
目的用逆转录病毒载体介导的基因转移方法,建立稳定表达人巨细胞病毒（HCMV）UL49基因的HELF细胞系。方法首先应用RT-PCR从感染HCMV的H......
目的：将人特异表达的癌胚抗原（ＣＥＡ）－ｃＤＮＡ克隆到逆转录病毒质粒载体ｐＬＸＳＮ中，构建成真核表达重组质粒ｐＬＸＳＮ－ＣＥＡ，为进一步表达表达人ＣＥＡ的动物肿瘤细胞模型作准备。方......
<正> Introduction Ac quired immunodeficiency syndrome (AIDS) is the first Human Retrovirus. It neither curable nor vacci......
根据反义ＲＮＡ作用原理，以禽流感病毒（ＡＩＶ）Ｈ１４Ｎ５ ｃＤＮＡ全长及５’端２３５个ｂｐ为目的基因，反向插入反转录病毒载体 ｐＬＸＳＮ中，命名为 ｐＬＸＳＮ－ＮＰ及 ｐＬＸＳＮ－ＮＰ，用脂质体包裹重组质粒转染包装细胞 ......
目的包装出携5拷贝缺氧反应元件（5 HRE）增强子和癌胚抗原启动子（CEAp）联合调控的超抗原中毒性休克综合征毒素-1（TSST-1）基因的逆转录病毒......
目的制备重组BIG-3（BMP-2 induced gene 3 Kb，BIG-3）逆转录病毒并初步探讨其对人骨髓基质细胞生物学功能的影响。方法 将pGEX4T-2-BIG......
目的研究单纯疱疹病毒胸腺嘧啶核苷激酶(herpes simplex virus thymidine kinase, HSVtk)基因联合丙氧鸟苷(ganciclovir, GCV)治疗......
国内首次从捕自中国三个地区的猕猴分离到三株逆转录病毒,经ELISA、免疫荧光检查与SRV—1呈阳性反应。免疫蛋白印迹试验加以证实,......
目的：利用逆转录病毒介导的shRNA稳定沉默NF kappa B p65基因，观察沉默NF kappa B p65对HT29细胞药物敏感性的影响。方法：设计并合成......
Objective: To observe the mutagenicity of retrovirus and adenovirus as transgenic vectors to evaluate the safety of tran......
Objective: To explore the potentiality of retroviral etiology in human acute myeloid leukemia(AML). Methods: The express......
