The dissipation and residual levels of difenoconazole and azoxystrobin in pear and soil under field conditions were determined by high-performance liquid chromatography coupled with tandem mass spectrometry(HPLC–MS/MS).Limit of detection(LOD)of difenoconazole and azoxystrobin in pear and soil was estimated lower than 0.01 mg/kg,and limit of quantitation(LOQ)was 0.05 mg/kg.When the fortified level was 0.05-1 mg/kg,the average recoveries of difenoconazole and azoxystrobin were 79%-103%and 73%-81%,and the relative standard deviations(RSDs,n=5)were 1-7%and 4-13%.The dissipation rates of difenoconazole and azoxystrobin were described using first-order kinetics and its half-life ranged from 8 to 10 days in pear and 17 to 36 days in soil.325g/L difenoconazole·azoxystrobin SC was applied with dosages of 216.7 and 325mg/kg on the surface of pear and soil,the spray times were three and four,the interval to harvest was 14 days,the terminal residues of difenoconazole and azoxystrobin were below the Chinas maximum residue limit(MRL,0.5 mg/kg)in pear when measured 7 days after the final application,which suggested that the use of these fungicides were safe for humans.The results showed that the dissipation rate of difenoconazole and azoxystrobin in pear were much faster than under field condition that in soil.This study will give a suggestion for the reasonable use of azoxystrobin under different cultivate conditions and can also provide reference to set MRL value in pear in China.