This lecture will describe the recent development of nanobiotechnologies based on the nanofabrication, molecular nanotechnology, and nanomaterials for bioanalysis and biomedical application.The technologies allow us to develop novel methods to analyze several biomolecules, including DNA, RNA, protein, peptide, polysaccharide, and glycoprotein.Array of nano-pillar, which is 200 nm wide and 4000 nm tall, is successfully applicable to fast separation of DNA within 10-25 s.Nano-ball, fabricated by molecular nanotechnology, of which diameter is 50 nm, is developed and successfully applied for fast separation of DNA fragments from 100 bp to 15 kbp.Bacteria cellulose, which is 50 nm diameter biofiber, is found to be useful high sensitive detection of biomolecule based on the optical confined effect as well as fast analysis of biomolecules.The nano-biodevice is extremely useful for fast separation of protein samples from several kDa to 200 kDa within 15 seconds.Utilizing this technique, complex protein mixture extracted from human T cell line, lymphoblastic Jurkat cells, were separated within 15 s with high reproducibility.The methods we have taken appear suitable for biomedical and clinical applications, including SNPs analysis, mutation analysis, haplotyping, DNA diagnosis, protein expression analysis, immunoassay, protein disease marker detection, point-of-care analysis.The methods are also applicable to molecular imaging of cancer cell and possible photodynamic therapy of cancer.