【摘 要】
:
Hepatitis B virus (HBV) infection causes acute hepatitis B (AHB),chronic hepatitis B (CHB),liver cirrhosis (LC),and eventually hepatocellular carcinoma (HCC).Hepatitis B e antigen (HBeAg) may play a r
【机 构】
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State Key Laboratory of Virology and College of Life Sciences,Wuhan University,Wuhan 430072,China
【出 处】
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第十一届全国病毒学学术研讨会暨第六届武汉现代病毒学国际研讨会
论文部分内容阅读
Hepatitis B virus (HBV) infection causes acute hepatitis B (AHB),chronic hepatitis B (CHB),liver cirrhosis (LC),and eventually hepatocellular carcinoma (HCC).Hepatitis B e antigen (HBeAg) may play a role in chronic HBV infection and its presence in the serum generally indicates ongoing viral replication and disease progression.
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目的:构建出HEV Ch-S-1株的全长cDNA克隆,并转染至HepG2细胞并对其表达进行初步研究。方法:利用重叠PCR方法获得HEV Ch-S-1株全长PCR产物;构建体外转录载体pKS-HEV,成功获得HEV基因组RNA,并转染至HepG2细胞,通过RT-nPCR、套式PCR和间接免疫荧光实验检测病毒的表达。
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