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A reliable, sensitive and selective method was successfully developed to determine 15 different mycotoxins simultaneously in foods and feeds. In this method, the homogenized sample was first treated with gel permeation chromatography 0.996) of 15 mycotoxins were obtained within their respective linear ranges. The average recoveries for lower, intermediate, and high spiked levels ranged from 80.1％～95.5％ in company with RSD ranged from 10.5％～19.6％. The method not only represents many advantages including simple pre-treatment, good purification and high sensitivity, but also successfully fit the minimum limiting level requests from various countries including EU USA and Japan.