Analyzing gene expression profile in K562 cells exposed to sodium valproate using microarray combine

来源 :广东省遗传学会第九届代表大会暨学术研讨会 | 被引量 : 0次 | 上传用户:fj123521
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  To explore the mechanism underlying antileukaemia effect of sodium valproate, the growth and survival of the K562 cell line were investigated.Global profiles of gene expression in K562 cells exposed to sodium valproate were assessed and validated.The differentially expressed genes identified were further used to query the connectivity map database to retrieve a ranked list of compounds that act on the same intracellular targets as sodiumvalproate.A significant increase in cell apoptosis and a change in gene expression profile were observed in valproate-exposed K562 cells.The significant enrichment analysis of gene ontology terms for the differentially expressed genes showed that these genes were involved in many important biological processes.Eight differentially expressed genes involved in apoptosis were verified by quantitative real-time PCR.The connectivity map analysis showed gene expression profile in K562 cells exposed to sodium valproate was most similar to that of HDACi and PI3K inhibitors, suggesting that sodium valproate might exert antileukaemic action by inhibiting HDAC as well as inhibiting PI3K pathway.In conclusion, our data might provide clues to elucidate the molecular and therapeutic potential of VPA in leukaemia treatment, and the connectivity map is a useful tool for exploring the molecular mechanism of drug action.
其他文献
Dihydrofolate reductase (DHFR) is a household enzyme that is responsible for the NADPH-dependent conversion of dihydrofolate (DHF) to tetrahydrofolate (THF).The mechanism involves proton donation at N
会议
Alkyl, as a bioorthogonal chemical reporter, can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans.In addition to the clickable chemical reactivity
会议
Objective Aim to provide an effective genetic testing for thalassemia (thal) , we analyzed genotype α and β-globin, as well as the polymorphism of β-globin gene in Cantonese in recent years.Methods Us
目的 通过体外实验探讨铂耐药的上皮性卵巢癌细胞中是否存在Notch信号通路异常激活及EMT样表型的获得,并探讨γ-分泌酶抑制剂对化疗耐药EOC增殖、粘附、侵袭、转移等生物学行为的影响,为卵巢癌治疗提供新思路.方法 体外培养人卵巢浆液性囊腺癌细胞株SKOV3及其顺铂耐药株SKOV3/DDP,PCR检测其Notch1 mRNA表达量的差异,western-blot检测γ分泌酶抑制剂DAPT作用于SKO
目的 通过超声引导下介入性穿刺术获取胎儿附属物标本进行遗传性耳聋基因产前诊断,降低遗传性耳聋患儿的出生率.方法 孕11~13+6周孕妇采用超声引导下绒毛活检术抽取胎盘绒毛;孕18~24+6周孕妇在超声引导下抽取羊水,孕24周以上孕妇在超声引导下抽取脐血.应用短串重复序列连锁分析(STR)进行母血污染鉴别,遗传性耳聋基因芯片检测技术对GJB2、GJB3、SLC26A4和mtDNA12SrRNA四个耳
线粒体tRNA基因是原发性高血压(Essential hypertension,EH)的突变热点区.文章报道了3个携带线粒体tRNAGIn 4363T>C突变的、具有母系遗传特征的原发性高血压家系的分子遗传学特征.3个家系的临床数据分析表明,3个家系母系成员的外显率为23.5%,非母系成员的外显率为2%,具有统计学差异(P<0.01),呈现母系遗传特征.线粒体全序列分析显示,3位先证者均携带同质性
Background: Obtaining fetal DNA or RNA by either chorionic villus sampling (CVS) or amniocentesis is currently, the gold standard prenataldiagnosis.However, these invasive procedures carry risk of mis
会议
Background: The noninvasive prenatal diagnosis procedures that are currently used to detect genetic diseases do not achieve desirable levels of sensitivity and specificity.Recently, fetal methylated D
会议
Background: The aim of this study was to determine whether the concentration of disintegrin and metalloprotease proteinl2 (ADAM12) in first trimester maternal serum can be used as a marker for first-t
会议
Background: The free beta subunit of human chorionic gonadotropin (free beta-hCG) is an important serum marker for biochemical screening.Its weekly median value varies with ethnicity.Most of the fluor
会议