【摘 要】
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The ORF2 protein of hepatitis E virus (HEV) is essential for the attachment and cell entry of the virus, but their mechanisms are still unknown because of t
【机 构】
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National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University,
【出 处】
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2008病毒性肝炎成就与挑战国际研讨会(International Confernece on Viral Hepati
论文部分内容阅读
The ORF2 protein of hepatitis E virus (HEV) is essential for the attachment and cell entry of the virus, but their mechanisms are still unknown because of the lack of isolated virus and a reliable cell culture system.We have previously produced a particulate ORF2 protein-p239, which had good immunogenicity and reactivity, and has been confirmed to have a simulated structure compared with native viruses.Here we use p239 as a bait to study the interaction between HEV and cellular proteins.p239 was tagged with a calmodulin binding peptide (CBP), and expressed and purified from E.Coli.
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