【摘 要】
：
田间调查发现，玉米、高粱丝黑穗病症状表现复杂多样，其原因不清，成为病害的田间诊断及抗病育种中需要解决的问题。本文采用病菌形态学特征与病菌核糖体基因ITS序列比对分析，对我国不同地区、不同品种、不同症状表现的玉米、高粱丝黑穗病菌进行鉴定。结果表明，玉米、高粱丝黑穗病田间症状表现差异较大，但经测定的病菌冬孢子萌发特点、菌落形态特征及病菌rDNA-ITS序列均与孢堆黑粉菌(Sporisorium rei
【机 构】
：
辽宁省农业科学院植物保护研究所,沈阳 110161;沈阳农业大学植物保护学院,沈阳 110161
【出 处】
：
中国植物病理学会2014年学术年会
论文部分内容阅读
田间调查发现，玉米、高粱丝黑穗病症状表现复杂多样，其原因不清，成为病害的田间诊断及抗病育种中需要解决的问题。本文采用病菌形态学特征与病菌核糖体基因ITS序列比对分析，对我国不同地区、不同品种、不同症状表现的玉米、高粱丝黑穗病菌进行鉴定。结果表明，玉米、高粱丝黑穗病田间症状表现差异较大，但经测定的病菌冬孢子萌发特点、菌落形态特征及病菌rDNA-ITS序列均与孢堆黑粉菌(Sporisorium reilianum)的形态及生物学特征相符，并非属于其他黑粉菌种类。研究证实了我国北方玉米、高粱丝黑穗病出现多种症状均为孢堆黑粉菌侵染所致，并非其他病菌种类侵染造成，可能与品种基因型或当地气候等有关。
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