2. 您的位置
3. 首页
4. 会议论文
5. Unraveling the catalytic mechanism of ecDHFR using neutron and ultrahigh-resolution x-ray diffractio

Unraveling the catalytic mechanism of ecDHFR using neutron and ultrahigh-resolution x-ray diffractio

来源 :中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议 | 被引量 : 0次 | 上传用户：shuibizi

【摘 要】

：

Dihydrofolate reductase (DHFR) is a household enzyme involved in many biosyntheticpathways including purine synthesis.The enzymatic reaction involves converting dihydrofolate(DHF) to tetrahydrofolate

【作 者】

：

Qun Wan Andrey Kovalevsky Mark Wilson Brad Bennett Paul Langan Chris Dealwis 

【机 构】

：

College of Medicine, Yangzhou University Biology

【出 处】

：

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

【发表日期】

：

2014年3期

【关键词】

：

neutron crystallography protonation state xray and neutron joint refinement DHFR 

下载到本地 , 更方便阅读

下载此文 赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

Dihydrofolate reductase (DHFR) is a household enzyme involved in many biosyntheticpathways including purine synthesis.The enzymatic reaction involves converting dihydrofolate(DHF) to tetrahydrofolate (THF) that requires hydride transfer.

其他文献

Rotenone inhibits mTOR signaling by induction of reactive oxygen species leading to apoptosis of neu

Rotenone,an inhibitor of the mitochondrial electron transport chain complex,may induce neuronal apoptosis contributing to neurodegenerative diseases.

会议

RotenonemTORROSApoptosisNeuronal cell

Novel mechanisms of bitter tastant inducing mouse airway smooth muscle cell contraction and relaxati

The bitter tastant chloroquine activates bitter taste receptors (TAS2Rs) and signaling through G protein subtype Gβγ to increase intracellular Ca2+ in airway muscle cells,and this Ca2+ increase result

会议

tas2rbitter tastantairway musclenon-selection cation channelsairway hyperres

miR-449通过调控DNA损伤应答基因XPC影响膀胱尿路上皮癌疾病进展

膀胱癌是我国泌尿生殖系统最常见的肿瘤,其发病率和死亡率均居泌尿肿瘤首位.膀胱癌的演进是多阶段多基因参与的结果,其中DNA损伤应答过程的异常可通过影响细胞周期、凋亡和DNA修复等重要生命活动导致肿瘤基因组的高度不稳定,与肿瘤的发生、发展密切相关.

会议

膀胱尿路上皮癌miR-449XPC

GAS2维持慢性粒细胞白血病细胞生长的功能研究

慢性粒细胞白血病(CML)是正常造血干细胞获得性形成BCR-ABL融合基因而诱发的克隆性血液肿瘤.抑制BCR-ABL酪氨酸激酶的小分子化合物,如Imatinib mesylate能使慢性期患者得到有效缓解,但单一药物尚不能治愈该疾病;这表明深入研究CML致病分子机制、寻找新的治疗靶标仍是CML研究的重点.

会议

CMLcalpainbeta-cateninGAS2HNRPDL

Exogenous Taurine attenuates mitochondrial oxidative stress and endoplasmic reticulum stress in rat

Taurine,a conditional essential amino acid,plays a critical role in cardiovascular functions.Here we examined the effects of taurine on mitochondria and Endoplasmic Reticulum in rat cardiomyocytes dur

会议

TaurineTaurine TransporterGlucose DeprivationMitochondrial Oxidative StressE

Intramolecular Regulation of the WW Domain of HYPB/SETD2 for Its Sequestration by the Huntingtin Agg

Huntingtons disease (HD) is a progressive neurodegenerative disorder caused by thepolyglutamine (polyQ) expansion in huntingtin (Htt) protein, a ubiquitously expressed proteinof yet unknown function.G

会议

Huntingtin HYPB/SETD2 Intramolecular interaction Aggregate Sequestration Proline

The dynamics characteristics of AK1

To analyze the backbone dynamics of AK1, we performed 15N relaxation measurementsusing NMR spectroscopy.Totally,115 assigned residues were used except the residueswith overlapping signals that were no

会议

Akl dynamics

The structural basis of RKIP binding with its substrate Raf-11-147

Raft kinase inhibitor protein (RKIP) directly interacts with Rafl kinase, thereby regulatingthe Rafl/MEK/ERK pathway which is vital for cell growth and differentiation.However, little is known about t

会议

RKIP Rafl147aa Binding sites

Really interesting new gene finger protein 121 (RNF121) is anovel Golgi-localized membrane protein t

Really interesting new gene (RING) finger protein is a large protein family in the humangenome.And have very important functions in physiological activities and cancer development.The biological funet

会议

RING finger protein 121 RNF121 apoptosis RING domain Golgi apparatus

Super Spy variants implicate flexibility in chaperone action

Experimental study of the role of disorder in protein function is challenging.It has beenproposed that proteins utilize disordered regions in the adaptive recognition of their variousbinding partners.

会议

Spy Chaperone flexibility