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目的：建立测定人血清中头孢拉定浓度的高效液相色谱法（ＨＰＬＣ）。方法：血清经高氯酸处理后进样，采用Ｂｉｏ－Ｒａｄ－Ｃ１８不锈钢柱，以甲醇一醋酸盐缓冲液（２５∶７５，ｖ／ｖ）为流动相，茶碱为内标，在波长２５４ｎｍ处测定。结果：头孢拉定的线性范围为５～１００ｍｇ／Ｌ，平均回收率为（１００３±２３）％，日内、日间精密度ＲＳＤ均小于３％。结论：本法简便、可靠、适用于临床监测。
Objective: To establish a high performance liquid chromatography (HPLC) for the determination of cefradine in human serum. Methods: Serum was injected with perchloric acid solution. Bio-Rad-C18 stainless steel column was used with methanol-acetate buffer (25:75, v / v) as mobile phase and theophylline as internal standard. 254nm at the determination. Results: The linear range of cefradine was 5 ~ 100 mg / L with the average recovery of (1003 ± 23)%. The intra-day and inter-day RSD were less than 3%. Conclusion: This method is simple, reliable and suitable for clinical monitoring.