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MicroRNAs (miRNAs) are genome-encoded, small non-coding RNAs that play important functions in development, biotic and abiotic stress responses, and other processes.In this study, to examine the regulation of miRNA gene expression, we used bioinformatic program TSSP and PlantCARE database predict the core promoters of 440 miRNAs identified from a soybean degradome library and found cis-acting elements for 369 miRNAs.