Torulopsis glabrata CCTCC M202019,a kind of mutli-vitamin anxotroph strain,is a strain for pyruvate and tt-ketoglutarate production.Uracil availability is one of the essential precursors for transcription and could impact the following metabolic flux distribution.Most of the genes in glycolytic pathway were constitutively expressed.In a uracil auxotroph T.glabrata strain,the limited uracil would be primarily used for the transcription of the glycolytic pathways.