【摘 要】
：
Recombinant virus-like particles (VLPs) harbouring foreign epitopes at certain surface-exposed positions are highly immunogenic.Recently,we have demonstrated that hamster polyomavirns major capsid pro
【机 构】
：
Laboratory of Immunology Institute of Biotechnology, Vilnius Lithuania
【出 处】
：
2008中国深圳蛋白质和多肽科学大会
论文部分内容阅读
Recombinant virus-like particles (VLPs) harbouring foreign epitopes at certain surface-exposed positions are highly immunogenic.Recently,we have demonstrated that hamster polyomavirns major capsid protein VPl-derived VLPs well tolerated the insertion of foreign sequences of different size and origin at four predicted surface-exposed sites.The current study was aimed at evaluating VPl-derived VLPs as carriers for hybridoma technology to generate monoclonal antibodies (mAbs) of desired specificity.We have employed chimeric VLPs containing either short or large inserts:the 9 aminoacid (aa)-long CTL epitope of human mucin 1 and 120 aa-long segment of hantavirns nucleocapsid protein,respectively.Chimeric VLPs harbouring the 9 aa-long insert elicited an efficient B cell response against the insert and promoted the production of 2 insert-specific mAbs.
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