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Currently, one of the most popular strategies for qualitative and quantitative study of complex protein samples are mass spectrometry (MS) based shotgun strategy and proteolytic 18O labeling, which depend on trypsin catalyzed sequence specific cleavage or labeling reaction.Though has long been well adopted in qualitative and quantitative proteomics study, the main limitation of traditional solution based trypsin digestion and 18O labeling is the prolonged incubation time needed for complete digestion and highly efficient labeling, which largely limits the analysis throughput.