【摘 要】
：
Top-down and bottom-up strategies are widely adopted in proteome study.However, no matter by which means, the enzymatic digestion is indispensable so that p
【机 构】
：
Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dali
【出 处】
：
DaLian Internationall Symposia and Exhibition on Chromatogra
论文部分内容阅读
Top-down and bottom-up strategies are widely adopted in proteome study.However, no matter by which means, the enzymatic digestion is indispensable so that proteins could be identified by MS.Up till now, free solution digestion is commonly used for protein digestion, which suffers disadvantages such as rather long incubation time, high liability for enzyme autodigestion and disability to achieve on-line hyphenation with the separation and identification systems.Therefore, it is requisite to develop novel enzymatic reactors.In our recent work, various microenzymatic reactors were prepared by immobilizing trypsin on different substrates, such as packing materials and monoliths, which showed great potential in proteome study.
其他文献
A successful hyphenation of in-tube solid phase microextraction (SPME) and pressure-assisted capillary electrochromatography (pCEC) was developed by install
目的：:通过分析温州地区真菌性角膜炎患者的病原学、药敏、实验室检查及治疗方式等，进一步为真菌性角膜炎的诊疗及预后评估提供依据。方法：:回顾性研究。收集2017年1月至2019年1
Chromatography has long been a valuable tool for pharmaceutical process development, however, in recent years chromatography has taken on an expanded role a
Multiple constituents of Traditional Chinese Medicine (TCM) are usually responsible for the therapeutic effects.The analysis of one or two major constituent
An automatic system with sample introduction and multi-dimensional separation for nanoflow liquid chromatography-tandem mass spectrometric (μLC-MS/MS) anal
The ability to detect changes in patterns of protein expression between normal and abnormal cells or tissues is of great importance to the field of biomarke
Analytical approaches with multi-resolution are highly desirable for extracting more information of the components from a sample under analysis.Herein, a ne
十九世纪六十年代初,我是北京劳动人民文化宫工人文学创作的成员。当时在每周的星期日,文化宫都请著名作家为学员们讲辅导课,如老舍、冰心、赵树理、臧克家等,都给我们辅导过
Carbohydrates are the most abundant biological molecules, and are widely distributed in nature.They can be isolated from plants, animals, bacteria and fungu
Therapeutic proteins produced in biotechnology sources from either inside cell, or outside cell.Because the former which is expressed in Escherichia coli (E