【摘 要】
：
[目的]小菜蛾Plutella xylostella (L.)是十字花科蔬菜的重要害虫,目前对包括氯虫苯甲酰胺在内的多种杀虫剂都已产生了高水平的抗性.现有研究已经表明多个蛋白编码基因参与调控了小菜蛾对氯虫苯甲酰胺的抗药性,但有关氯虫苯甲酰胺抗药性转录后调控机制的报道还相对较少.本研究系统鉴定了与小菜蛾对氯虫苯甲酰胺抗药性相关的microRNA (miRNA).[方法]构建了一个小菜蛾敏感品系(CH
【机 构】
：
中国农业大学植保学院昆虫学系,北京,100193
论文部分内容阅读
[目的]小菜蛾Plutella xylostella (L.)是十字花科蔬菜的重要害虫,目前对包括氯虫苯甲酰胺在内的多种杀虫剂都已产生了高水平的抗性.现有研究已经表明多个蛋白编码基因参与调控了小菜蛾对氯虫苯甲酰胺的抗药性,但有关氯虫苯甲酰胺抗药性转录后调控机制的报道还相对较少.本研究系统鉴定了与小菜蛾对氯虫苯甲酰胺抗药性相关的microRNA (miRNA).[方法]构建了一个小菜蛾敏感品系(CHS)以及两个氯虫苯甲酰胺抗性品系(CHR,ZZ)的miRNA文库,并进行高通量测序分析,运用生物信息学软件对miRNA及其靶基因进行预测.[结果]通过测序共鉴定得到了481个已知的小菜蛾miRNAs和30个新的miRNAs.在CHR和CHS之间共检测到31个差异表达的miRNAs,在ZZ和CHS之间检测到176个差异表达的miRNAs,其中有21个差异表达的miRNA是两个抗性品系共有的.利用miRNA靶标预测软件miRanda和RNAhybrid,在186个差异表达的miRNA中共预测到了2802个靶标基因,其中包含了多条解毒酶基因.进一步应用qRT-PCR对部分差异表达的miRNA及其靶标基因的表达情况进行了验证.[结论]本研究首次系统的鉴定了小菜蛾氯虫苯甲酰胺抗性相关的miRNA,为进一步在小菜蛾和其他目标昆虫中研究miRNA介导的氯虫苯甲酰胺抗性机制提供了重要线索.
其他文献
Transcription factors and epigenetic modifications plays an important role of gene expression,and also some more experimental phenomena have been discovered this phenomenon.However,in the experiment,T
It is known that chromatin three-dimensional (3D) structure plays important roles in nucleus.Recently,the extensive works from chromosome conformation capture (3C) derived techniques,such as Hi-C,have
In recent years,the research on lncRNA is increasing sharply,cumulative reports have suggested that lncRNA is involved in many biological processes of diseases such as cancer.Here,we present a compreh
Histone modifications play critical roles in regulating the expression of developmental genes during embryo development in mammals.Here,by using a small-scale chromatin immunoprecipitation sequencing
N6-methyladenosine (m6A) is the most prevalent and abundant modification in mRNAs that has been linked to many key biological processes.High-throughput experiments have generated m6A-peaks across the
A systematic transcriptome survey is essential for the characterization and comprehension of the molecular basis underlying phenotypic variations.Recently developed RNA-seq methodology has facilitated
Through alternative processing ofpre-RNA,mammalian gene often generates multiple mRNAs and protein isoforms.It is increasingly recognized that disruption of normal programs of splicing regulated by di
Nucleosome dynamics,facilitating histone exchange and increasing nucleosomal DNA accessibility,is an underexplored feature of chromatin-based transcriptional regulation in addition to histone marks,su
褐飞虱(Nilaparvata lugens)是重要的水稻害虫之一.长期依赖化学防治导致褐飞虱对不同类型杀虫剂产生了不同水平的抗性.明确田间褐飞虱对新烟碱类杀虫剂吡虫啉产生高水平抗性的代谢机理,能够为害虫抗药性综合治理提供支持.本研究基于褐飞虱基因组信息,通过比较室内抗性品系、田间抗性种群,以及吡虫啉诱导前后的敏感品系中,解毒代谢酶P450s的表达量差异,分析了与吡虫啉代谢相关的P450s基因或基