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Served as a model organism for elucidate photosynthetic mechanism, more and more attentions were paid to the biosynthesis and regulation of photosynthetic pigments in purple bacteria.This work compared the difference in carotenoid compositions of five purple bacteria under semiaerobic-dark and anaerobic-light condition.The results showed that strain R7 and YL75 could not grow under semiaerobic-dark conditions, the other three strains showed red colonies.Colonies color under light-anaerobic condition was changed when exposed to air, especially for strain XL, its colony color was changed from green-yellow into red.