New generation sequencing techniques can provide very informative insights into the transcriptome,such as expression of gene isoforms and fusion transcripts.However,the currently available transcriptome analysis tools are for SGS short reads,the unreliable alignments of which can introduce bias and even errors in downstream analysis.While the recent application of Third Generation Sequencing (TGS) long reads,such as PacBio data,to human transcriptome analysis has greatly advanced the field,key bioinformatic analysis platforms are missing.