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应用改进的ＩＥＦ电泳方法，对中国２６个省、市（区）的８９５份大豆资源进行脂肪氧化酶缺失类型鉴定；对ＩＥＦ电泳图谱进行激光扫描分析，比较品种间各条同工酶带活性差异；研究脂肪氧化酶的缺失与籽粒颜色、百粒重、播种期类型及生育期的关系；通过生物体系超弱发光动力学分析，研究种子发芽前后脂肪氧化酶缺失体活性动态变化。结果表明：在中国大豆资源中发现了４种脂肪氧化酶缺失体类型，鉴定出４３份缺失突变体，占鉴定总数的４．８０％；脂肪氧化酶的缺失与籽粒颜色和播种期类型有关，其中褐色、黑色大豆缺失频率较高；萌动种子发芽前，正常大豆的超弱发光强度明显高于脂肪氧化酶缺失体的发光强度，探索用超弱发光强度鉴别脂肪氧化酶缺失体的可能性。此外，还研究制定了一套快速、简便的大豆脂肪氧化酶鉴定技术，为种质创新研究及开发食品资源提供鉴定方法。
The improved IEF electrophoresis method was used to identify the type of lipoxygenase deficiency in 895 soybean resources of 26 provinces and cities in China. The IEF electrophoresis pattern was analyzed by laser scanning. The relationship between the loss of lipoxygenase and the color of kernel, the weight of 100 kernels, the type of sowing period and the growth period were studied. The dynamic changes of the activity of the lipoxygenase-deficient body before and after seed germination were studied by the ultraweak luminescence kinetic analysis of the biological system. The results showed that four types of lipoxygenase deletion bodies were found in soybean resources of China, and 43 deletion mutants were identified, accounting for 4.80% of the total number of identification. The loss of lipoxygenase was related to the color of seeds and the type of sowing date, Among them, the frequency of deletion of brown and black soybean was higher. Before weed germination, the weakest luminescence intensity of normal soybean was obviously higher than the luminescence intensity of lipoxygenase deficient body, and the possibility of identifying lipoxygenase-deficient body by ultra-weak luminous intensity was explored. In addition, a set of rapid and simple soybean lipoxygenase identification technology has also been researched and developed to provide identification methods for germplasm innovation research and development of food resources.