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A recombinant transposon Tn5-GmSp, produced by replacing the central BglⅡ fragmentwith a BamHⅠ one of R1033, was utilized to detect Tn5 transposition inhibition in Rhizo-bium leguminosarum. Two types of suicide plasmids (pXS102 and pCW3/pCW30) were con-st