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采用内皮素建立培养的血管平滑肌细胞增殖模型，用氚－胸腺嘧啶核苷（［３Ｈ］－ＴｄＲ）参入法，流式细胞术，Ｗｅｓｔｅｒｎ及Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交方法，观察了１－（２，６－二甲基苯氧基）－２－（３，４－二甲氧基苯乙胺基）丙烷盐酸盐（ＤＤＰＨ）对血管平滑肌细胞增殖的作用及对热应激蛋白７０ｋｄ（ＨＳＰ７０）及其ｍＲＮＡ和抑癌基因Ｐ５３表达的影响。结果发现：ＤＤＰＨ能逆转内皮素所致的［３Ｈ］－ＴｄＲ参入量增多，阻止血管平滑肌细胞由静止期（Ｇ０／Ｇ１期）进入ＤＮＡ合成期（Ｓ期）和有丝分裂期（Ｇ２／Ｍ期），并能逆转内皮素引起的ＨＳＰ７０及ｍＲＮＡ表达增强，Ｐ５３抑癌基因及ｍＲＮＡ表达减弱。提示：ＤＤＰＨ能抑制血管平滑肌细胞增殖，与ＨＳＰ７０及Ｐ５３的调控有关。
The cultured vascular smooth muscle cell proliferation model was established by using endothelin. The effects of 1- (2,6-bis Methylphenoxy) -2- (3,4-dimethoxyphenylethylamino) propane hydrochloride (DDPH) on the proliferation of vascular smooth muscle cells and the effects on heat shock protein 70kd (HSP70) and its mRNA And tumor suppressor gene P53 expression. The results showed that DDPH could reverse the increase of the [3H] -TdR incorporation induced by endothelin and prevent the smooth muscle cells from entering the DNA synthesis phase (S phase) and the mitotic phase (G2 / M phase) from quiescent phase (G0 / G1 phase) , And can reverse endothelin-induced HSP70 and mRNA expression increased, P53 tumor suppressor gene and mRNA expression decreased. Tip: DDPH can inhibit the proliferation of vascular smooth muscle cells, and the regulation of HSP70 and P53.