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目的:观察六味地黄丸对手机频率电磁辐射雄性大鼠睾丸组织形态学、氧化损伤和细胞凋亡的影响。方法:30只雄性SD大鼠随机分为正常组、辐射组、六味地黄丸组,每组各10只。正常组不辐射,辐射组和六味地黄丸组大鼠每天暴露于900 MHz手机频率辐射4 h共18 d;六味地黄丸组大鼠每日灌胃六味地黄丸悬液(1 ml/100 g体重),其他组大鼠每天灌胃等量纯净水。实验结束后腹腔麻醉取材处死大鼠,分别取睾丸组织固定和冷冻,常规HE染色观察组织形态学变化,比色法检测睾丸组织丙二醛(MDA)、谷胱甘肽(GSH)水平,免疫组化法观察睾丸细胞bax,bcl-2蛋白表达的平均光度值。结果:与正常组相比,辐射组大鼠睾丸生精小管细胞结构松散,精母细胞层数减少,内有空洞出现;睾丸组织MDA水平显著升高[(0.36±0.07)μmol/g prot vs(0.25±0.06)μmol/g prot,P<0.05],GSH水平显著降低[(42.10±5.08)mg/g prot vs(52.29±4.16)mg/g prot,P<0.05];辐射组大鼠睾丸生精细胞bax蛋白表达显著增强(0.91±0.10 vs 0.21±0.01,P<0.01),bcl-2蛋白表达显著减弱(0.18±0.07 vs 0.70±0.94,P<0.01)。六味地黄丸组大鼠睾丸组织形态学接近正常,与辐射组比较,睾丸组织MDA含量显著降低[(0.30±0.05)μmol/g prot,P<0.05],GSH含量无明显变化[(45.34±5.21)mg/g prot,P>0.05],睾丸生精细胞bax蛋白表达(0.41±0.05)明显减弱(P<0.01),bcl-2蛋白表达(0.27±0.08)明显增强(P<0.01)。结论:六味地黄丸可改善手机频率电磁辐射造成的大鼠睾丸组织结构损伤,降低睾丸组织氧化损伤,减少睾丸细胞凋亡。
Objective: To observe the effect of Liuweidihuangwan on testicular histomorphology, oxidative damage and apoptosis of male rats exposed to electromagnetic field with cellphone frequency. Methods: Thirty male SD rats were randomly divided into normal group, radiation group and Liuweidihuangwan group, with 10 rats in each group. Radiation group and Liuweidihuangwan group were exposed to 900MHz cellphone radiation daily for 4 hours for 18 hours. The rats in Liuweidihuangwan group were treated with liweidihuangwan suspension (1 ml / 100 g body weight ), Other groups of rats fed with the same amount of purified water daily. After the experiment, the rats were sacrificed by intraperitoneal anesthesia. The testicular tissues were fixed and frozen respectively. The histopathological changes were observed by HE staining. The content of malondialdehyde (MDA) and glutathione (GSH) Histochemistry was used to observe the average photometric value of bax and bcl-2 protein expression in testicular cells. Results: Compared with the normal group, the testicular seminiferous tubule cells in the radiation group were loosely structured and the number of spermatocytes was decreased, with voids inside. The MDA level in testis tissue was significantly increased [(0.36 ± 0.07) μmol / g prot vs (0.25 ± 0.06) μmol / g prot, P <0.05], and GSH levels were significantly lower in [42.10 ± 5.08 mg / g prot vs 52.29 ± 4.16 mg / g prot, P < The expression of bax protein in spermatogenic cells was significantly increased (0.91 ± 0.10 vs 0.21 ± 0.01, P <0.01), while the expression of bcl-2 protein was significantly decreased (0.18 ± 0.07 vs 0.70 ± 0.94, P <0.01). Compared with radiation group, the content of MDA in testis tissue was significantly decreased [(0.30 ± 0.05) μmol / g prot, P <0.05], and there was no significant change in GSH content [(45.34 ± 5.21 ) mg / g prot, P> 0.05]. The expression of bax protein in testis spermatogenic cells was significantly decreased (0.41 ± 0.05) and the expression of bcl-2 protein was significantly increased (P <0.01). Conclusion: Liuweidihuangwan can improve the damage of testicular tissue structure induced by electromagnetic radiation of cell phone, reduce the oxidative damage of testicular tissue and reduce the apoptosis of testicular cells.