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为了研究 HUMCSF1PO、HUMTPOX和 HUMTHO1基因座在云南省基诺族、佤族和布朗族中的遗传多态性分布及其在法医学中的应用价值 ,采用 PCR扩增和 PAGE变性胶电泳分离技术 ,对三个少数民族 346名无关个体及家系进行了分析 ,并将其应用于法医学实践。在这三个群体中 HU MCSF1PO、HUMTPOX和HUMTHO1基因座分别检出了 7个、8个和 6个等位基因 ,基因型频率分布均符合 Hardy- Weinberg平衡 ,三基因座累计个人识别率在 0 .9889～ 0 .9947之间 ,累计非父排除率为 0 .6 716～ 0 .7497;家系调查证实其等位基因的传递遵循孟德尔遗传规律。并对三个基因座在法医学中的应用价值也作了评价。结果提示三个基因座在三个少数民族中多态性分布较好 ,可用于法医学个人识别和亲权鉴定。
In order to study the genetic polymorphism distribution of HUMCSF1PO, HUMTPOX and HUMTHO1 loci in Jinuo, Wa and Blang ethnic groups in Yunnan province and their application value in forensic medicine, PCR amplification and PAGE denaturing gel electrophoresis 346 ethnic minorities unrelated individuals and their families were analyzed and applied to forensic practice. Seven, eight and six alleles were detected in the HU MCSF1PO, HUMTPOX and HUMTHO1 loci in all three populations. The genotype frequency distributions were all in accordance with the Hardy-Weinberg equilibrium, and the cumulative three-locus individuals recognition rate was 0 .9889 ~ 0.9947, the cumulative non-parent exclusion rate was 0.676 ~ 0.7449; pedigree investigation confirmed that the transmission of alleles followed Mendelian inheritance. The application value of three loci in forensic medicine was also evaluated. The results suggest that the three loci distribution of polymorphisms in the three ethnic minorities better, can be used for forensic personal identification and paternity testing.