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目的探讨白介素-6(IL-6)基因-572C/G多态性和白介素-10(IL-10)基因-1082A/G多态性与小儿热性惊厥(FS)的关系。方法选取2013年1月-2013年6月我院FS患儿50例作为实验组,选取同期50名健康体检儿童作为对照组。应用酶联免疫法分别检测血清中IL-6与IL-10的含量,聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)检测IL-6基因-572C/G位点和IL-10基因-1082A/G位点的基因型。分析血清中IL-6与IL-10的含量水平和基因型频率与小儿热性惊厥相关性。结果实验组血清IL-6含量明显高于对照组,而IL-10含量低于对照组。实验组患儿IL-6基因-572位点基因分布频率分别为CC42%,CG36%,GG22%,与对照组比较CC型和GG型均有显著差异。实验组IL-10基因-1082位点基因分布频率分别为AA46%,AG38%,GG16%,与正常对照组比较AA型和GG型均有显著差异。结论 IL-6和IL-10的基因多态性与FS密切相关,有助于FS发病机制和病因的临床研究,在遗传学方面有一定的指导意义。
Objective To investigate the relationship between -572C / G polymorphism of interleukin-6 (IL-6) gene and -1082A / G polymorphism of interleukin-10 (IL-10) gene and febrile convulsion (FS) in children. Methods From January 2013 to June 2013, 50 children with FS in our hospital were selected as the experimental group and 50 healthy children in the same period were selected as the control group. Serum levels of IL-6 and IL-10 were detected by enzyme-linked immunosorbent assay. The -572C / G locus of IL-6 gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Genotype of IL-10 gene -1082A / G site. The levels of IL-6 and IL-10 in serum and genotype frequency were analyzed in relation to febrile seizures in children. Results The level of serum IL-6 in the experimental group was significantly higher than that in the control group, while the content of IL-10 in the experimental group was lower than that in the control group. The distribution frequency of IL-6 gene-572 locus in experimental group was CC42%, CG36%, GG22%, respectively. There was significant difference between CC group and GG group in control group. The frequency of -1082 site of IL-10 gene in experimental group was AA46%, AG38%, GG16%, respectively. There was significant difference between AA group and GG group in the experimental group. Conclusion The genetic polymorphisms of IL-6 and IL-10 are closely related to FS and contribute to the clinical study of the pathogenesis and etiology of FS, which may be of guiding significance in genetics.