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目的:探讨碱性成纤维生长因子(bFGF)基因修饰的自体骨髓间充质干细胞(BMSCs)促进大鼠下颌牵引成骨的作用。方法:36只雄性SD大鼠,随机分为实验组和对照组,同时对两组大鼠右下颌骨进行牵引成骨,牵引期最后1 d,实验组大鼠牵引间隙内注射转染重组质粒pcDNA-bFGF的BMSCs,对照组注射转染pcDNA空质粒的BMSCs。分别于固定期第2,4,8周分3批处死,并进行放射学检查、组织学检查及骨密度检测。结果:两组牵引间隙内均有新骨形成。各时间点实验组牵引间隙内新骨的形成和骨质密度均较对照组高(P<0.05)。结论:bFGF基因修饰的BMSCs可有效促进DO新骨形成,为颌面部骨缺损的重建提供了一个新的修复方法。
Objective: To investigate the effect of basic fibroblast growth factor (bFGF) gene-modified autologous bone marrow mesenchymal stem cells (BMSCs) in promoting the mandibular distraction osteogenesis in rats. Methods: Thirty-six male Sprague-Dawley rats were randomly divided into experimental group and control group. At the same time, the right mandibles of both groups were distracted and induced for the last 1 days. The rats in experimental group were injected with the recombinant plasmid pcDNA-bFGF BMSCs, control group injected with pcDNA empty plasmid BMSCs. The animals were sacrificed in 3 batches in the 2nd, 4th, and 8th weeks of the fixed period respectively, and the radiological, histological and bone mineral density tests were performed. RESULTS: New bone was formed in both traction gaps. At each time point, the formation of new bone and bone density in the traction gap of the experimental group were higher than those in the control group (P <0.05). CONCLUSION: BMSCs modified with bFGF gene can effectively promote the formation of DO new bone, providing a new method for the reconstruction of maxillofacial defects.