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目的:研究携带小鼠IL-10基因的重组腺病毒表达载体修饰的CD4+T细胞对哮喘小鼠气道NF-κB表达的影响,从而探讨IL-10治疗哮喘的可能分子机制。方法:BALB小鼠随机分成6组,正常对照组(A组)、哮喘模型组(B组)I、L-10基因修饰CD4+T细胞治疗组(C组)、LacZ基因修饰的CD4+T细胞治疗组(D组)、未经任何基因修饰的CD4+T细胞治疗组(E组)、生理盐水治疗组(F组),应用卵白蛋白激发的哮喘模型。应用流式细胞仪分离小鼠外周血CD4+T细胞,基因转染采用重组腺病毒表达载体。应用West-ernblot分析肺气道的IκB的表达。结果:IL-10基因转染的CD4+T细胞在第7天表达IL-10最高,并可以持续维持高水平表达40d左右。C组与B组和F组IκB表达经统计学处理差异均具有显著性(P<0.05)。结论:IL-10基因转染的CD4+T细胞使哮喘小鼠气道IκB表达上升,提示IL-10治疗哮喘可能通过抑制NF-κB传导有关。
Objective: To study the effect of CD4 + T cells modified by recombinant adenovirus carrying mouse IL-10 gene on the expression of NF-κB in airway of asthmatic mice to explore the possible molecular mechanism of IL-10 in treating asthma. Methods: BALB mice were randomly divided into 6 groups: normal control group (group A), asthma model group (group B), L-10 gene modified CD4 + T cell treatment group (group C), LacZ gene modified CD4 + T Cell treatment group (group D), CD4 + T cell group without any gene modification (group E), saline treatment group (group F), and ovalbumin-challenged asthma model. CD4 + T cells were isolated from peripheral blood of mice by flow cytometry, and the gene was transfected into the recombinant adenovirus vector. Western-ernblot was used to analyze the expression of IκB in lung airways. Results: The IL-10 gene transfected CD4 + T cells expressed the highest level of IL-10 on the 7th day, and could maintain high level expression for about 40 days. The difference of IκB expression between group C and group B and group F was statistically significant (P <0.05). Conclusion: IL-10 gene transfected CD4 + T cells in asthmatic mice airway IκB expression increased, suggesting that IL-10 treatment of asthma may be related to the inhibition of NF-κB conduction.