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目的:研究玉郎伞多糖(YLS)、玉郎伞黄酮(YF)与六月青多糖(LYQPS)联合对四氯化碳(carbon tetrachloride,CCl4)所致大鼠肝纤维化的干预作用及机制。方法:Wistar大鼠随机分成两组,模型组大鼠采用50%CCl4花生油溶液2mL·kg-1ig造模,共7周,正常组用生理盐水代替。将病理检查确认肝纤维化已形成的Wistar大鼠40只,随机分成4组,分别给予相应药物进行干预,每日1次,连续4周。末次给药24h后处死大鼠,采集肝组织,检测大鼠肝组织中羟脯氨酸(Hyp)、超氧化物歧化酶(SOD)、丙二醛(MDA)的含量,采用HE染色观察肝组织纤维化程度。结果:联合用药高剂量(540mg·kg-1)组中MDA(10.43±0.95)nmol·mg-1(P<0.01)和Hyp(0.26±0.05)μg·mg-1(P<0.05)含量明显下降,SOD(39.19±6.00)U·mg-1(P<0.05)活力显著升高;低剂量(270mg·kg-1)组有一定的保护肝脏组织的作用。各药物组肝组织细胞纤维化程度均有所减轻。结论:YLS,YF,YQPS联合用药可减轻CCl4诱导的肝纤维化。
AIM: To investigate the effects of YLS, YF and LYQPS on hepatic fibrosis in rats induced by carbon tetrachloride (CCl4) and its mechanism . Methods: Wistar rats were randomly divided into two groups. The rats in the model group were treated with 2 mL · kg-1 of 50% CCl4 peanut oil solution for 7 weeks. The normal rats were replaced by normal saline. Pathological examination confirmed the formation of liver fibrosis in 40 Wistar rats were randomly divided into four groups were given the appropriate drug intervention, once daily for 4 weeks. The rats were sacrificed 24h after the last administration, and the liver tissues were collected to detect the content of Hyp, SOD and MDA in liver tissue of rats, and the liver was observed by HE staining Degree of tissue fibrosis. Results: The levels of MDA (10.43 ± 0.95) nmol · mg-1 (P <0.01) and Hyp (0.26 ± 0.05) μg · mg-1 (P <0.05) were significantly higher in the high dose of 540mg · kg- (39.19 ± 6.00) U · mg-1 (P <0.05). The low dose (270mg · kg-1) group had certain protective effect on the liver tissue. The degree of liver fibrosis in each drug group was alleviated. Conclusion: Combination of YLS, YF and YQPS can reduce CCl4-induced hepatic fibrosis.