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目的了解深圳市吸毒人群和异性性传播感染者HIV-1的分子流行病学情况。方法应用BigDye链终止反应试剂盒,对2000-2008年收集的62例深圳市吸毒人群和61例异性性传播感染者HIV-1RNA2.6-kbgag-RT区的核苷酸序列进行测定,并确定其基因型分布。结果样品中共存在CRF07BC,CRF08BC,CRF01AE三种重组株和B,B′,C三种亚型。在吸毒人群HIV-1中,CRF07BC和CRF08BC是主要基因型,在样品中的比例为37.1%(23/62)和45.2%(28/62),CRF01AE、B′、B、C亚型所占比例分别为9.7%(6/62)、4.8%(3/62)、1.6%(1/62)和1.6%(1/62)。同样,异性性传播HIV-1感染者的基因型也以CRF07BC和CRF08BC为主,其比例为29.5%(18/61)和41.0%(25/61),而CRF01AE、B′、B亚型分别为21.3%(13/61)、6.6%(4/61)、3.3%(2/61),无C亚型存在。结论深圳市异性性传播HIV-1感染者中流行的病毒基因型主要来自于当地吸毒人群。
Objective To understand the molecular epidemiology of HIV-1 among drug users and heterosexual transmission in Shenzhen. Methods The nucleotide sequence of HIV-1RNA2.6-kbgag-RT region in 62 HIV-infected persons and 61 HIV-1RNA collected from 2000 to 2008 collected in Shenzhen from 2000 to 2008 was determined by using BigDye chain termination reaction kit. Its genotype distribution. Results There were three subtypes CRF07BC, CRF08BC and CRF01AE and three subtypes B, B and C in the sample. Among HIV-1 drug users, CRF07BC and CRF08BC were the major genotypes, accounting for 37.1% (23/62) and 45.2% (28/62) of the samples, respectively. CRF01AE, B ’, B and C subtypes accounted for The proportions were 9.7% (6/62), 4.8% (3/62), 1.6% (1/62) and 1.6% (1/62), respectively. Similarly, the genotypes of HIV-1 infected with heterosexual transmission were also predominantly CRF07BC and CRF08BC, with a proportion of 29.5% (18/61) and 41.0% (25/61), respectively, while CRF01AE, B ’and B subtypes 21.3% (13/61), 6.6% (4/61), 3.3% (2/61), no subtype C exists. Conclusion The prevalence of HIV genotypes in heterosexually transmitted HIV-1 in Shenzhen mainly comes from local drug addicts.