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精子和 T 淋巴细胞具有共同抗原,取自精子免疫性不孕患者的血清有 T 细胞的凝集抗体,而且其体内循环 T 淋巴细胞低于正常。本文对52对企图怀孕而2～10年不孕的夫妇的血清、精液、宫颈粘液和阴道分泌物进行精子细胞毒抗体(C-Sp)和淋巴细胞细胞毒抗体(C-Ly)的测定。受试的妇女身体和排卵正常,无输卵管阻塞及生殖道感染。男性精子数目、活性和形态正常。每个试验用健康、有生殖力的夫妇为对照。用标记二乙酰荧光素的精子在有补体存在的情况下与连续稀释的 C-Sp 的标本反应,用溴化乙锭对精子第二次染色后,用荧光显微镜测定每个稀释度杀死精子的百分数,用此来测定
Sperm and T lymphocytes have a common antigen, taken from the serum of patients with sperm immune infertility T cell agglutination antibodies, and the body’s circulating T lymphocytes below normal. In this study, 52 sperm, semen, cervical mucus and vaginal secretions from couples who were trying to become pregnant for 2 to 10 years were tested for sperm cytotoxic antibody (C-Sp) and lymphocyte cytotoxic antibody (C-Ly). The women tested had normal body and ovulation without tubal obstruction and genital tract infection. Male sperm count, activity and morphology were normal. Each test used healthy, fecundity couples as controls. Sperm labeled with diacetyl fluorescein is reacted with serial dilutions of C-Sp in the presence of complement, sperm are stained with ethidium bromide for a second time, and each dilution is assayed by a fluorescence microscope to kill sperm The percentage, using this to determine