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目的:从蜱类媒介中分离斑点热群立克次体。方法:用鸡胚卵黄囊培养法直接分离。结果:自福建省宁化县林区麂体表捉获的越原血蜱(Haemephysalisyeni)中分离到1株斑点热群立克次体,经微量补结试验和PCR检测初步确定为西伯利亚立克次体(Ricketsiasibirica)。结论:进一步从病原学上证明了福建省存在北亚斑点热自然疫源地的可能性
OBJECTIVE: To isolate spotted fever group Rickettsia from tick vectors. Methods: Chick embryo yolk sac culture method directly. Results: One spotted fever group Rickettsia was isolated from Haemephysalisyeni collected from the forest muntjac body in Ninghua County, Fujian Province. The results showed that Siberian Rick Ricketsiasibirica. Conclusion: The possibility of existence of the northern natural hot spots in northern Fujian province is further proved etiologically