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对下丘脑神经分泌细胞的研究,以往多用Nissl法和Gomori法,但这两种方法都不够敏感,且不能将加压素细胞同含其他神经分泌物(如催产素)的细胞区分开来,这在需要专一对含加压素或催产素的细胞之形态、分布与功能的研究中就显得不足了。免疫细胞化学方法则克服了上述方法的不足,而且还能显示一些用传统的神经解剖学方法所未显示的结构。然而,目前所用的免疫细胞化学方法多为使用石腊切片或较薄的冰冻切片染色的,它们虽能显示神经元的胞体与突起,但在同一切片上追踪范围有限,较难反映神经元与神经元之间的相互关系,因此,将特异的免疫细胞化学方法同较厚的切片相结合来研究下丘脑的一些肽类神经元的形态、肽类物质的含量、神经元与神经元之间的关系及神经通路是人们感兴趣的课题,在这方面,国内尚未见报道。
Previous studies of neurosecretory cells in the hypothalamus have used the Nissl method and the Gomori method. However, neither method is sensitive enough and can not distinguish vasopressin cells from cells containing other neurological secretions (such as oxytocin) This is not enough in a study that requires a specific study of the morphology, distribution and function of vasopressin or oxytocin-containing cells. The immunocytochemistry method overcomes the deficiencies of the above methods and shows some structures that are not shown by traditional neuroanatomy methods. However, most immunocytochemistry methods used at present are stained with paraffin sections or thin frozen sections. Although they can show cell bodies and processes of neurons, they have limited tracing range on the same section and are difficult to reflect the difference between neurons and Therefore, specific immunocytochemical methods combined with thicker sections to study the morphology of some peptide neurons in the hypothalamus, the content of peptides, the relationship between neurons and neurons The relationship and neural pathways are the subjects of interest. In this respect, there is no report yet in China.