论文部分内容阅读
目的 探讨 p2 7与p16基因产物在口腔鳞癌和癌旁组织中的表达 ,了解 p2 7与 p16在口腔鳞癌发生中的意义。方法 采用 p2 7与 p16单克隆抗体 ,用免疫组化S -P法对 3 7例口腔鳞癌组织、3 0例癌旁组织、10例正常口腔黏膜 p2 7与p16的表达进行检测 ,结果 p2 7与 p16表达减少与口腔鳞癌的发生和恶性程度有关。p2 7表达下降与临床分期有关 ,p16与临床分期无关。p2 7与 p16在口腔鳞癌中表达无关 ,但在口腔鳞癌中表达随恶性程度的增高而降低。结论 p2 7与 p16蛋白有可能作为有价值的标志物 ,在口腔鳞癌的诊断、治疗、预后判断中起辅助作用。
Objective To investigate the expression of p27 and p16 gene in oral squamous cell carcinoma and adjacent non-cancerous tissues and to investigate the significance of p27 and p16 in the pathogenesis of oral squamous cell carcinoma. Methods The p27 and p16 monoclonal antibodies were used to detect the expression of p27 and p16 in 37 cases of oral squamous cell carcinoma, 30 cases of para-carcinoma and 10 cases of normal oral mucosa by immunohistochemical S-P method. Results p2 7 and p16 expression decreased with the occurrence of oral squamous cell carcinoma and the degree of malignancy. p27 decreased expression and clinical stage, p16 and clinical stage unrelated. The expression of p27 was not related to the expression of p16 in oral squamous cell carcinoma, but it decreased with the increase of malignant degree in oral squamous cell carcinoma. Conclusion The p27 and p16 proteins may serve as valuable markers in the diagnosis, treatment and prognosis of oral squamous cell carcinoma.