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血管活性肠肽(VIP)是一种28肽。首先在猪小肠中发现。免疫组织化学技术表明VIP也存在于中枢神经系统不同部位,包括下丘脑内侧基底部(MBH),提示它可能是一种作用于此处的神经递质。已知VIP可使垂体前叶释放催乳素(PRL),但它在促性腺激素释放中的作用尚存在着争议。本文用体外双室灌流系统检验了VIP对下丘脑分泌促性腺激素释放激素(GnRH)与对垂体分泌黄体生成素(LH)的影响。实验用Wistar大鼠(体重200～250g)。分为两组,一组大鼠在动情周期的动情间期断头,二组大鼠切除卵巢两周后行断头术。切出MBH与垂体,用连续双室灌流系统进行灌流。MBH放入第一个
Vasoactive intestinal peptide (VIP) is a 28 peptide. First found in pig intestine. Immunohistochemistry shows that VIP is also present in different parts of the central nervous system, including the hypothalamic medial basal ganglia (MBH), suggesting that it may be a neurotransmitter acting here. VIP is known to release prolactin (PRL) from the anterior pituitary, but its role in gonadotropin release remains controversial. In this study, we examined the effect of VIP on the secretion of gonadotropin-releasing hormone (GnRH) from hypothalamus and the secretion of luteinizing hormone (LH) from pituitary by an in vitro two-compartment perfusion system. Experimental Wistar rats (body weight 200 ~ 250g). Divided into two groups, a group of rats in the estrous cycle of estrus decapitation, two groups of rats ovariectomy after two weeks. MBH and pituitary gland were excised and perfusion was performed with a continuous double chamber perfusion system. MBH into the first one