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为明确耿氏硬草Pseudosclerochloa kengiana(Ohwi)Tzvel潜在抗性种群对不同乙酰辅酶A羧化酶(ACCase)类除草剂的抗性水平及其靶标抗性的分子机制,采用剂量-反应曲线法测定了耿氏硬草对精鰁唑禾草灵、炔草酯、烯禾啶、烯草酮和唑啉草酯5种ACCase类除草剂的抗性水平,扩增并比对了耿氏硬草抗性和敏感种群间ACCase基因的差异。结果显示:与敏感种群SD-6相比,耿氏硬草种群SD-32对精鰁唑禾草灵、炔草酯、烯禾啶、烯草酮和唑啉草酯产生了不同水平的抗性,抗性倍数分别为16.5、7.5、15.0、4.4和5.7;SD-32种群ACCase基因CT区域的2078位氨基酸基因由GAT突变为GGT,导致天冬氨酸(Asp)被甘氨酸(Gly)取代。分析表明,ACCase基因2078位氨基酸的突变可能是导致耿氏硬草对ACCase类除草剂产生抗性的重要原因之一。
In order to understand the molecular mechanism of resistance to various acetyl-CoA carboxylase (ACCase) herbicide resistant potential populations of Pseudosclerochloa kengiana (Ohwi) Tzvel and to investigate the molecular mechanism of its target resistance, the dose-response curve The resistance of the hardwoods to five ACCase herbicides, fenvalerate, clodinafop propargyl, cetirizine, clethion and pinoxaden, were amplified and compared with that of the hardwood Differences of ACCase Genes between Resistant and Sensitive Populations. The results showed that SD-32 had different levels of resistance to fenvalerate, clodinafop propargyl, cetirizine, clethion and pinoxaden as compared with susceptible population SD-6 The resistance and multiplicity of resistance were 16.5, 7.5, 15.0, 4.4 and 5.7, respectively. The 2078 amino acid sequence in the CT region of SD-32 population was changed from GAT to GGT, resulting in the substitution of Asp by Gly . The analysis showed that the mutation of the amino acid at position 2078 of ACCase gene may be one of the important reasons for the resistance of the hardwood to ACCase herbicides.