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目的:研究六味地黄丸水提液体外培养人牙周膜成纤维细胞增殖的影响。方法:以体外培养的人牙周膜成纤维细胞为实验基础,六味地黄丸水提液分组:药物浓度为0.001、0.01、0.1、1、10mg/ml,对照组药物浓度为0mg/ml,分别作用1、2、3、4、5天。用MTT法检测六味地黄丸水提液对体外培养人牙周膜成纤维细胞增殖的影响。结果:六味地黄丸水提液在浓度0.001-0.1mg/ml范围内对人牙周膜细胞增殖有促进作用,而1、10mg/ml时对细胞增殖表现抑制作用,与对照组比较差异有统计学意义(P<0.05)。结论:六味地黄丸水提液在一定药物浓度范围内能够促进体外培养人牙周膜成纤维细胞增殖。
Objective: To study the effect of Liuwei Dihuang pill aqueous extract on proliferation of human periodontal ligament fibroblasts in vitro. Methods: Human periodontal ligament fibroblasts were cultured in vitro. The aqueous extract of Liuweidihuangwan was divided into groups: drug concentration of 0.001,0.01,0.1,1,10mg / ml and control group of drug concentration of 0mg / ml, respectively Role 1,2,3,4,5 days. Effect of Liuwei Dihuang Pill Extract on Proliferation of Human Periodontal Ligament Fibroblasts Cultured in. Results: The aqueous extract of Liuweidihuangwan could promote the proliferation of human periodontal ligament cells in the concentration range of 0.001-0.1mg / ml, while the inhibitory effect of 1,10mg / ml on the proliferation of the cells was significant compared with the control group Significance (P <0.05). Conclusion: Liuweidihuangwan aqueous extract can promote the proliferation of human periodontal ligament fibroblasts in a certain concentration range.