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本研究旨在了解体外高渗钠环境下粒细胞集落刺激因子(G-CSF)对吞噬细胞活性的作用。取志愿者40ml全血分成两组,每组4份,每份5ml,分别置入140、180、220和260mmol/L盐水中制成细胞悬液。在实验组试管中加入稀释成0.1ml生理盐水的150ng重组人粒细胞克隆刺激因子,在3713环境下培育60分钟后,用流式细胞仪测定被荧光剂标记的粒细胞吞噬活性。结果显示,在140mmol/L钠浓度下对照
This study aimed to understand the effect of granulocyte-colony stimulating factor (G-CSF) on the activity of phagocytes in vitro in hypertonic sodium environment. Take volunteers 40ml whole blood divided into two groups, each 4 copies, each 5ml, were placed 140,180,220 and 260mmol / L saline made of cell suspension. 150ng of recombinant human granulocyte colony stimulating factor diluted to 0.1ml of physiological saline was added to the experimental group test tube and incubated at 3713 for 60 minutes. Then the phagocytic activity of granulocytes labeled with the fluorescent agent was measured by flow cytometry. The results showed that the control was at 140 mmol / L sodium concentration