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目的:探讨迷走神经兴奋对内毒素血症大鼠血浆肿瘤坏死因子-α(TNFα)含量的影响。方法:雄性Wistar 大鼠,乌拉坦腹腔注射麻醉,左颈总动脉插管,记录平均动脉压及心率,分离并切断双侧颈迷走神经,静注脂多糖(LPS)后持续刺激左侧迷走神经远端20min,间隔20min 后重复,共刺激3次。随机分为手术对照、LPS 对照、迷走神经刺激和迷走神经切断后静注 LPS 等7组。分别于静注 LPS 后1h、1.5h 和2h 测定血浆 TNFα、皮质醇及丙氨酸氨基转移酶(ALT)。结果:假手术组血浆中 TNFα含量水平较低,静注 LPS 各组 TNFα含量升高8～24倍,高峰均在1.5h 出现,并于2h 回落;迷走神经刺激组血浆 TNFα含量较单纯 LPS 组和迷走神经切断后注射LPS 组明显降低;单纯注射 LPS 组血浆皮质醇含量在2h 与其余各组相比增高明显;迷走神经刺激组血浆 ALT含量显著低于单纯 LPS 组。结论:提示迷走神经兴奋能显著降低内毒素血症大鼠体内促炎细胞因子的产生,减轻其全身炎症反应。
Objective: To investigate the effect of vagus nerve excitability on the level of plasma tumor necrosis factor-α (TNFα) in endotoxemia rats. Methods: The male Wistar rats were anesthetized with urethane and the left common carotid artery was cannulated. The mean arterial pressure and heart rate were recorded. The bilateral cervical vagal nerves were isolated and severed. The distal vagal nerve was stimulated by intravenous injection of lipopolysaccharide (LPS) 20min, repeated 20min intervals, costimulation 3 times. Randomly divided into surgical control, LPS control, vagal nerve stimulation and vaginal nerve transection after intravenous LPS and other 7 groups. Plasma TNFα, cortisol and alanine aminotransferase (ALT) were measured at 1h, 1.5h and 2h after LPS injection. Results: The levels of TNFα in the plasma of sham operation group were lower than those in the LPS group and the TNFα level in the LPS group was 8 ~ 24 times higher than that in the LPS group LPS group was significantly decreased after injection of vagotomy; plasma cortisol level in LPS group increased significantly compared with the other groups at 2h; plasma ALT level in vagus nerve stimulation group was significantly lower than that in LPS group. Conclusion: It is suggested that excitability of the vagus nerve can significantly reduce the production of proinflammatory cytokines in rats with endotoxemia and reduce the systemic inflammatory response.