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目的 观察微波辐照后大鼠脑海马、皮层神经细胞线粒体超微病理变化与mitochondrialtranscriptionfactorA(mtTFA)mRNA表达 ,探讨mtTFA在线粒体能量代谢中的调控作用。方法 微波急性辐照大鼠 1h后 ,应用电镜观察大鼠脑海马与皮层神经细胞线粒体超微病理结构 ,应用RT PCR检测mtTFAmRNA表达。结果 与相应对照组比较 ,3mW cm2 微波辐照后即刻及 3、2 4h ,脑海马与皮层神经细胞线粒体超微结构无明显变化 ,mtTFAmRNA表达差异无显著性 (P >0 .0 5 )。而 30mW cm2 微波辐照后即刻及 3、2 4h ,可见脑海马、皮层神经细胞线粒体肿胀、偶见嵴排列紊乱、嵴稀疏、断裂等病理现象 ;脑海马与皮层mtTFAmRNA表达均高于对照组 ,差异有显著性 (P <0 .0 1) ,辐照后即刻及 3、2 4h与对照组相比 ,海马mtTFAmRNA表达分别升高了 6 7.0 0 %、80 .0 0 %、30 .0 0 % ,皮层mtTFAmRNA表达分别升高了 133.0 0 %、86 .0 0 %、2 33.0 0 % ;并且微波急性辐照后脑海马与皮层相应组比较 ,mtTFAmRNA表达量差异有显著性 (P <0 .0 1)。结论 小剂量 3mW cm2 微波辐照对海马和皮质神经细胞线粒体结构损伤不明显 ,30mW cm2 微波急性辐照后神经细胞线粒体已发生结构功能改变 ,皮质损伤较海马重 ;mtTFA对线粒体能量代谢具有一定的调节作用。
Objective To observe the ultrastructural changes of mitochondria and the mRNA expression of mitochondrial transcription factor A (mtTFA) in the hippocampus and cortex of rats after microwave irradiation and to explore the role of mtTFA in mitochondrial energy metabolism. Methods After acute microwave irradiation for 1 h, the ultrastructure of mitochondria in hippocampal and cortical neurons of rats was observed by electron microscope. The mtTFA mRNA expression was detected by RT PCR. Results Compared with the corresponding control group, the mitochondrial ultrastructure of neurons in hippocampus and cortex did not change significantly immediately after 3mW cm2 microwave irradiation and at 3 and 24h. There was no significant difference in mtTFA mRNA expression (P> 0.05). However, the mitochondrial swelling in hippocampus and cortical neurons was observed immediately after exposure to 30 mW cm2 microwave and 3,24 h after exposure to microwave radiation. The sparse cristae were sparsely arranged and sparsely cristae were found. The expression of mtTFA mRNA in hippocampus and cortex were higher than those in control group, (P <0.01). Compared with the control group, the mRNA expression of mtTFA in the hippocampus increased by 6 7.0%, 80.0% and 30.0%, respectively % And cTTFA mRNA expression in cortex increased by 133.0%, 86.0% and 23.0% respectively. Compared with the corresponding group, the expression of mtTFA mRNA in hippocampus and cerebral cortex significantly increased after microwave irradiation (P <0. 0 1). Conclusion The damage of mitochondria in hippocampus and cortical neurons was not obvious under low dose of 3mW cm2 microwave irradiation. The structure and function of mitochondria of neurons changed after 30mW cm2 of microwave irradiation, and the damage of cortex was heavier than that of hippocampus. However, mtTFA had certain mitochondrial energy metabolism Regulatory effect.