“抗病值”在大豆抗孢囊线虫病遗传研究中应用的探讨

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以抗病值表示大豆单株对孢囊线虫的抗性,探讨其在大豆对孢囊线虫4号生理小种抗性遗传 研究中的应用。在所研究的4个高感×高抗杂交组合中,直接用孢囊数作为抗性的参数,不分离群体 具有较大的方差,群体平均数与方差有较强的正相关;而采用抗病值表示抗性,不分离群体有较小的 方差,但F_2分离群体方差较大。与原始数据相比,经平方根转化后,单株孢囊数的群体平均数与方差 的高度正相关只有轻微的下降;而单株抗病值的群体平均数与方差的相关性则明显降低。4个组合单 株抗病值的广义遗传力为57.49%~71.79%,高于单株孢囊数的48.42%~65.96%;根据抗病值推导 出的最小基因数目为3~4,比用孢囊数推导为2~3的结果更接近按质量性状遗传估算出的结果。对 F_2单株频次分布研究表明,采用抗病值标准品种法分级统计的高抗株数,与按全国抗性分级标准下< 10个孢囊/株的株数完全一致,而且得到了更广泛的中间类型分布。按质量性状遗传模式对4个高感 x高抗组合F_2分离群体研究表明,灰皮支黑豆和元钵黑豆对SCN4号生理小种的抗性遗传可能由三 对隐性基因和二对显性基因控制。 The disease resistance value of the soybean plant cyst nematode resistance, to explore its soybean cyst nematode 4 physiological race resistance genetic research. Among the four highly susceptible × high resistant hybrid combinations studied, the number of cysts was used directly as the parameter of resistance, the non-segregating population had greater variance, and the population mean was strongly and positively correlated with variance. However, The disease value showed resistance, but the segregation population did not have the smaller variance, but the F 2 segregation population had larger variance. Compared with the original data, after the square root transformation, there was only a slight positive correlation between the population average number of individual cysts and the variance of variance. However, the correlation between population mean value of individual disease resistance values ​​and variance was significantly decreased. The general heritability of resistance value of four combinations was 57.49% ~ 71.79%, which was higher than that of single cyst, 48.42% ~ 65.96%. The smallest gene The number of 3 to 4, than the number of cysts deduced to 2 to 3 results closer to the quality of the genetic estimates of the results. The study on frequency distribution of F_2 plants showed that the number of high resistant plants classified by the anti-disease value standard method was completely consistent with the number of <10 cysts / plant under the national resistance classification standard and was more broadly intermediate Type distribution. According to the genetic trait of quality traits, four high susceptibility and high resistance combinations F 2 populations were studied. The results showed that the resistance inheritance of SCN 4 race could be determined by three pairs of recessive genes and two pairs of dominant Gene control.
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